CYPP10 is a kit for extracting nuclear and cytoplasmic protein from cultured cells or fresh tissue. Cultured cells can be separated in about 90 minutes. The extracted proteins are non-denatured and active, suitable for Western blot, EMSA, footprinting, reporter gene assays and enzyme activity assays.
Catalog no. CYPP10 · For research use only
Key features
- Separates nuclear and cytoplasmic protein from cultured cells or fresh tissue in about 90 minutes
- Cells swell in hypotonic conditions, the membrane is broken to release cytoplasmic protein, and nuclei are then extracted with a high-salt reagent
- Non-denatured, active protein for Western, EMSA, footprinting, reporter gene and enzyme assays
- 50 preps at 2 x 10^6 HeLa cells (about 40 mg) per sample; scalable
- Fresh tissue only; for research use only
Catalog numbers and pack sizes
| Catalog # | Product | Pack size |
|---|---|---|
| CYPP1001 | Nuclear and Cytoplasmic Protein Extraction Kit | 50 preps |
Specifications
| Format | Extraction kit |
|---|---|
| Sample | Cultured cells or fresh tissue |
| Capacity | 50 samples of 2 x 10^6 HeLa cells (~40 mg) |
| Time | About 90 minutes (cultured cells) |
| Protein state | Non-denatured, active |
| Quantification | BCA assay (CYPP01); not suitable for Bradford |
| Use | Research use only |
Applications
- Western blot of nuclear and cytoplasmic fractions
- EMSA and footprinting
- Reporter gene and enzyme activity assays
Ordering
Single-unit orders are accepted. Quotes and invoices are in USD with payment by company bank transfer, and different items can be combined into one shipment. Send the catalog number, quantity and destination via the quote form.
Nuclear and Cytoplasmic Protein Extraction Kit FAQ
What do I need to add myself?
PMSF, which must be added 2-3 minutes before the extraction reagent goes onto the sample since it breaks down quickly in water. Work on ice or at 4 °C.
Can I use frozen tissue?
It is only suited to fresh tissue; frozen tissue extracts poorly, and fewer than 50 tissue samples can usually be processed.
How do I measure protein concentration?
Use the BCA kit (CYPP01). The extracts are not suitable for the Bradford method.
How do I order CYPP10?
Request a quote with the catalog number; single-unit orders accepted, payment in USD by company transfer, and items can be combined into one shipment.
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