CYPP08 is a reversible protein stain for nitrocellulose or PVDF membranes, used to check transfer efficiency before Western blot. It stains protein blue-green with sensitivity below 25 ng, about 10 times that of Ponceau S (250 ng), and gives no background. Binding is mainly reversible ionic binding, so downstream Western blot is not affected. Nitrocellulose is recommended, as it usually stains better than PVDF.
Catalog no. CYPP08 · For research use only
Key features
- No background; sensitivity below 25 ng, about 10 times that of traditional Ponceau S
- Bands stain blue-green, easier to photograph than Ponceau red
- Reversible: protein properties and antibody-antigen binding unaffected; compatible with Western blot, mass spectrometry and N-terminal sequencing
- Confirms transfer success and roughly checks equal loading across lanes
- Simple, quick procedure; research use only
Catalog numbers and pack sizes
| Catalog # | Product | Pack size |
|---|---|---|
| CYPP0801 | Super-Sensitive Reversible Membrane Staining Kit | 100 mL |
| CYPP0802 | Super-Sensitive Reversible Membrane Staining Kit | 200 mL |
Specifications
| Format | Reversible membrane stain |
|---|---|
| Membranes | Nitrocellulose (recommended) or PVDF |
| Sensitivity | <25 ng |
| Band colour | Blue-green |
| Compatibility | Western blot, mass spectrometry, N-terminal sequencing |
| Use | Research use only |
Applications
- Checking protein transfer to nitrocellulose or PVDF before Western blot
- Estimating whether lane loading is roughly equal
- Documenting transfer with photographs
Ordering
Single-unit orders are accepted. Quotes and invoices are in USD with payment by company bank transfer, and different items can be combined into one shipment. Send the catalog number, quantity and destination via the quote form.
Super-Sensitive Reversible Membrane Staining Kit FAQ
Does it affect the Western blot afterwards?
No. Staining is reversible and does not affect protein properties or antibody-antigen binding.
Which membrane is better?
Nitrocellulose is recommended; staining is usually better than on PVDF.
How sensitive is it?
Below 25 ng, about 10 times more sensitive than Ponceau S.
How do I order CYPP08?
Request a quote with the catalog number; single-unit orders accepted, payment in USD by company transfer, and items can be combined into one shipment.
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