CYPL21 is a spin-column plasmid midiprep kit with a high-yield SDS-alkaline lysis that increases yield 1-2 times. It removes most endotoxin and other bacterial components and yields 100-500 μg from 30-70 mL of culture.
Catalog no. CYPL21 · For research use only
Key features
- High-yield buffer formulation increases yield 1-2 times
- 100-500 μg high-copy plasmid from 30-70 mL LB culture
- Removes most endotoxin; suited to protoplast and general transfection
- Protein removal solution clears nucleases, including from JM series and HB101
- No toxic phenol or chloroform and no ethanol precipitation; research use only
Catalog numbers and pack sizes
| Catalog # | Product | Pack size |
|---|---|---|
| CYPL2101 | HighPure Plasmid Midi Kit (Low Endotoxin) | 20 preps |
Specifications
| Format | Spin column, silica membrane |
|---|---|
| Yield | 100-500 μg from 30-70 mL LB culture |
| Endotoxin | Low (most removed) |
| Scale | Midi |
| Use | Research use only |
Applications
- Protoplast and general transfection
- PCR and digestion
- Transformation
Ordering
Single-unit orders are accepted. Quotes and invoices are in USD with payment by company bank transfer, and different items can be combined into one shipment. Send the catalog number, quantity and destination via the quote form.
HighPure Plasmid Midi Kit (Low Endotoxin) FAQ
What yield is typical?
100-500 μg from 30-70 mL LB culture.
For difficult cell lines?
An endotoxin-free midi or maxi plasmid kit is advised for especially demanding transfection.
Does it handle nuclease-rich strains?
Yes, including JM series and HB101.
How do I order CYPL21?
Request a quote with the catalog number; single-unit orders accepted, payment in USD by company transfer, and items can be combined into one shipment.
Browse related products: Rna & dna extraction kits.