CYPL20 is a solution-type kit for large plasmids such as BAC, PAC, P1 and cosmid DNA, which spin columns handle poorly because of size and low copy number. Alkaline lysis and an endotoxin removal reagent clear protein, polysaccharide, endotoxin and RNA with a few simple centrifugations.
Catalog no. CYPL20 · For research use only
Key features
- Typical yield 30-50 μg BAC DNA from 150 mL LB culture, 80-90% recovery
- Endotoxin below 0.1 EU/μg DNA; typical OD260/OD280 around 1.8
- No column, phenol or chloroform; avoids shearing of large DNA on membranes
- Solution reagents can be scaled proportionally for mini, midi or maxi preps
- Concentration up to 3 μg/μL; research use only
Catalog numbers and pack sizes
| Catalog # | Product | Pack size |
|---|---|---|
| CYPL2001 | BAC/PAC Large Plasmid Kit (Solution Type, Endotoxin-Free) | 20 preps |
Specifications
| Format | Solution-based, no column |
|---|---|
| Yield | 30-50 μg BAC DNA from 150 mL LB culture |
| Recovery | 80-90% |
| Endotoxin | Below 0.1 EU/μg DNA |
| Typical OD260/OD280 | About 1.8 |
| Use | Research use only |
Applications
- BAC/PAC/P1/cosmid preparation
- Cell transfection
- Sequencing and library work
Ordering
Single-unit orders are accepted. Quotes and invoices are in USD with payment by company bank transfer, and different items can be combined into one shipment. Send the catalog number, quantity and destination via the quote form.
BAC/PAC Large Plasmid Kit (Solution Type, Endotoxin-Free) FAQ
Why not use a standard spin column?
Large plasmids over 100 kb are low-copy and can be sheared by passing through a column membrane.
Can the protocol be scaled?
Yes, solution reagents can be scaled proportionally for mini, midi or maxi preps.
What endotoxin level is reported?
Below 0.1 EU/μg DNA.
How do I order CYPL20?
Request a quote with the catalog number; single-unit orders accepted, payment in USD by company transfer, and items can be combined into one shipment.
Browse related products: Rna & dna extraction kits.