CYPL14 is a solution-type, column-free plasmid kit for large-scale, transfection-grade DNA, including large plasmids and BAC/PAC. Alkaline lysis and an endotoxin removal reagent clear protein, polysaccharide, endotoxin and RNA with a few simple centrifugations.
Catalog no. CYPL14 · For research use only
Key features
- 0.4-1 mg high-copy plasmid from 150-200 mL LB culture, 80-90% recovery
- Endotoxin below 0.1 EU/μg DNA; typical OD260/OD280 around 1.8
- No column, phenol or chloroform; late steps run in microcentrifuge tubes
- Gentle on large plasmids, including BAC/PAC above 100 kb if alkaline lysis can extract them
- Concentration up to 3 μg/μL; research use only
Catalog numbers and pack sizes
| Catalog # | Product | Pack size |
|---|---|---|
| CYPL1401 | PhaseIso BAC DNA Kit | 20 preps |
Specifications
| Format | Solution-based, no column |
|---|---|
| Yield | 0.4-1 mg from 150-200 mL LB culture |
| Recovery | 80-90% |
| Endotoxin | Below 0.1 EU/μg DNA |
| Typical OD260/OD280 | About 1.8 |
| Use | Research use only |
Applications
- Cell transfection
- BAC and large plasmid preparation
- Digestion, transformation, PCR and sequencing
Ordering
Single-unit orders are accepted. Quotes and invoices are in USD with payment by company bank transfer, and different items can be combined into one shipment. Send the catalog number, quantity and destination via the quote form.
PhaseIso BAC DNA Kit FAQ
Is a column used?
No, purification uses simple centrifugation steps.
What endotoxin level is reported?
Below 0.1 EU/μg DNA.
Can very large plasmids be purified?
Yes, over 100 kb including BAC/PAC, if alkaline lysis can extract them.
How do I order CYPL14?
Request a quote with the catalog number; single-unit orders accepted, payment in USD by company transfer, and items can be combined into one shipment.
Browse related products: Rna & dna extraction kits.