CYPL13 is a spin-column endotoxin-free plasmid maxiprep kit. After modified SDS-alkaline lysis, a special endotoxin removal agent binds endotoxin by centrifugation, and plasmid DNA is purified on a silica membrane.
Catalog no. CYPL13 · For research use only
Key features
- Endotoxin below 0.1 EU/μg DNA
- 0.5-2 mg high-copy plasmid from 100-300 mL LB culture, over 80% recovery
- Protein removal solution clears nucleases, including from JM series and HB101
- No toxic phenol or chloroform and no ethanol precipitation
- Usable for transfection, digestion, transformation, PCR, in vitro transcription and sequencing; research use only
Catalog numbers and pack sizes
| Catalog # | Product | Pack size |
|---|---|---|
| CYPL1301 | EndoFree Plasmid Maxi Kit | 10 preps |
Specifications
| Format | Spin column, silica membrane |
|---|---|
| Endotoxin | Below 0.1 EU/μg DNA |
| Yield | 0.5-2 mg from 100-300 mL LB culture |
| Scale | Maxi |
| Use | Research use only |
Applications
- Eukaryotic cell transfection
- Large-scale plasmid preparation
- Digestion, PCR, sequencing
Ordering
Single-unit orders are accepted. Quotes and invoices are in USD with payment by company bank transfer, and different items can be combined into one shipment. Send the catalog number, quantity and destination via the quote form.
EndoFree Plasmid Maxi Kit FAQ
What endotoxin level is reported?
Below 0.1 EU/μg DNA.
What yield is typical?
0.5-2 mg from 100-300 mL LB culture.
Is phenol needed?
No.
How do I order CYPL13?
Request a quote with the catalog number; single-unit orders accepted, payment in USD by company transfer, and items can be combined into one shipment.
Browse related products: Rna & dna extraction kits.