CYPL11 is a spin-column plasmid maxiprep kit using a modified SDS-alkaline lysis. Plasmid DNA binds a silica membrane at high salt and low pH, and 0.5-2 mg can be obtained from 100-300 mL of culture.
Catalog no. CYPL11 · For research use only
Key features
- 0.5-2 mg high-copy plasmid from 100-300 mL LB culture, recovery about 80%
- High yield, high supercoiled proportion and good purity
- No toxic phenol or chloroform and no ethanol precipitation
- DNA usable for digestion, transformation, PCR, in vitro transcription and sequencing
- Research use only
Catalog numbers and pack sizes
| Catalog # | Product | Pack size |
|---|---|---|
| CYPL1101 | Plasmid Maxi Kit | 10 preps |
Specifications
| Format | Spin column, silica membrane |
|---|---|
| Yield | 0.5-2 mg from 100-300 mL LB culture |
| Recovery | About 80% |
| Scale | Maxi |
| Use | Research use only |
Applications
- Large-scale plasmid preparation
- Restriction digestion
- Transformation
- PCR, in vitro transcription and sequencing
Ordering
Single-unit orders are accepted. Quotes and invoices are in USD with payment by company bank transfer, and different items can be combined into one shipment. Send the catalog number, quantity and destination via the quote form.
Plasmid Maxi Kit FAQ
What yield is typical?
0.5-2 mg from 100-300 mL LB culture.
What recovery is reported?
About 80%.
Is phenol or chloroform needed?
No.
How do I order CYPL11?
Request a quote with the catalog number; single-unit orders accepted, payment in USD by company transfer, and items can be combined into one shipment.
Browse related products: Rna & dna extraction kits.