CYPL02 is a spin-column plasmid miniprep kit that yields plasmid DNA in 8 minutes using a modified SDS-alkaline lysis. Plasmid DNA binds a silica membrane under high-salt, low-pH conditions and is eluted in low-salt, high-pH buffer.
Catalog no. CYPL02 · For research use only
Key features
- Modified SDS-alkaline lysis for fast preparation
- Suited to low-nuclease strains such as XL-1 Blue, Top10 and DH5α
- For nuclease-rich endA strains (JM series, HB101) or wild-type strains, a high-purity plasmid mini kit (CYPL03) is advised
- No toxic phenol or chloroform and no ethanol precipitation
- Plasmid DNA usable for digestion, transformation, transfection, PCR, in vitro transcription and sequencing; research use only
Catalog numbers and pack sizes
| Catalog # | Product | Pack size |
|---|---|---|
| CYPL0201 | 8-Minute Plasmid Mini Kit | 50 preps |
| CYPL0202 | 8-Minute Plasmid Mini Kit | 100 preps |
| CYPL0203 | 8-Minute Plasmid Mini Kit | 200 preps |
Specifications
| Format | Spin column, silica membrane |
|---|---|
| Method | Modified SDS-alkaline lysis |
| Time | About 8 minutes |
| Strains | XL-1 Blue, Top10, DH5α |
| Use | Research use only |
Applications
- Restriction digestion
- Transformation
- General transfection
- PCR
- In vitro transcription and sequencing
Ordering
Single-unit orders are accepted. Quotes and invoices are in USD with payment by company bank transfer, and different items can be combined into one shipment. Send the catalog number, quantity and destination via the quote form.
8-Minute Plasmid Mini Kit FAQ
How long does a prep take?
About 8 minutes.
Which strains is it for?
Low-nuclease strains such as XL-1 Blue, Top10 and DH5α.
What for nuclease-rich strains?
Use CYPL03 for JM series, HB101 and other endA or wild-type strains.
How do I order CYPL02?
Request a quote with the catalog number; single-unit orders accepted, payment in USD by company transfer, and items can be combined into one shipment.
Browse related products: Rna & dna extraction kits.