CYEP06 is GoldenView, a nucleic acid dye that can replace ethidium bromide (EB) in agarose electrophoresis. Sensitivity is comparable to EB and use is identical: add 5 μL to 100 mL of agarose gel solution. Under UV transillumination double-stranded DNA shows green fluorescence, and it can also stain RNA.
Catalog no. CYEP06 · For research use only
Key features
- Replaces ethidium bromide with comparable sensitivity and the same usage
- Add 5 μL per 100 mL agarose gel solution
- Double-stranded DNA fluoresces green under UV; also stains RNA
- For DNA recovery, cut bands in daylight to avoid UV and EB damage and improve downstream cloning, transformation and transcription
- Keep gels 0.5 cm thick or less; wear gloves; for research use only
Catalog numbers and pack sizes
| Catalog # | Product | Pack size |
|---|---|---|
| CYEP0601 | GoldenView Nucleic Acid Stain | 1 mL |
Specifications
| Format | Nucleic acid gel stain |
|---|---|
| Use level | 5 μL per 100 mL gel |
| Sensitivity | Comparable to EB |
| Fluorescence | Green (dsDNA, UV) |
| Gel thickness | 0.5 cm or thinner |
| Use | Research use only |
Applications
- Agarose gel staining of DNA and RNA
- Gel recovery of DNA fragments under visible light
- Replacing EB in routine electrophoresis
Ordering
Single-unit orders are accepted. Quotes and invoices are in USD with payment by company bank transfer, and different items can be combined into one shipment. Send the catalog number, quantity and destination via the quote form.
GoldenView Nucleic Acid Stain FAQ
How much do I add?
5 μL per 100 mL of agarose gel solution, the same way EB is used.
Any handling advice?
Keep gels no thicker than 0.5 cm. Repeated re-melting of stained gels has a minor effect on sensitivity. Dye safety is debated, and it can irritate skin and eyes, so wear gloves, wash after contact and dispose of waste properly.
Why use it for DNA recovery?
Bands can be cut in natural light, avoiding UV and EB damage to the DNA and improving later cloning, transformation and transcription efficiency.
How do I order CYEP06?
Request a quote with the catalog number; single-unit orders accepted, payment in USD by company transfer, and items can be combined into one shipment.
Browse related products: Research reagents.