CYDN22 is a spin-column kit for lambda phage genomic DNA. Lambda lysate supernatant is first treated with an RNase A/DNase I enzyme mix to remove residual host DNA and RNA, phage are pelleted, lysed with SDS, and the cleared lysate is bound to a silica membrane.
Catalog no. CYDN22 · For research use only
Key features
- Works with liquid culture lysates and solid culture plates
- Typical yield about 10 μg phage DNA from 10 mL lysate supernatant
- A single sample usually completed within 1.5 hours
- No toxic phenol and no ethanol precipitation
- Typical OD260/OD280 of 1.7-1.9; DNA suited to direct digestion and sequencing; research use only
Catalog numbers and pack sizes
| Catalog # | Product | Pack size |
|---|---|---|
| CYDN2201 | Lambda Phage Genomic DNA Kit | 50 preps |
| CYDN2202 | Lambda Phage Genomic DNA Kit | 100 preps |
Specifications
| Format | Spin column, silica membrane |
|---|---|
| Sample | Lambda phage lysate (liquid or plate) |
| Yield | About 10 μg per 10 mL lysate |
| Time | About 1.5 hours |
| Typical OD260/OD280 | 1.7-1.9 |
| Use | Research use only |
Applications
- Library clone sequencing
- Restriction digestion
- Lambda DNA preparation
Ordering
Single-unit orders are accepted. Quotes and invoices are in USD with payment by company bank transfer, and different items can be combined into one shipment. Send the catalog number, quantity and destination via the quote form.
Lambda Phage Genomic DNA Kit FAQ
What yield is typical?
About 10 μg from 10 mL lambda lysate supernatant.
How is host DNA removed?
With an RNase A/DNase I mix before phage lysis.
Can plate lysates be used?
Yes, both liquid lysates and solid culture plates.
How do I order CYDN22?
Request a quote with the catalog number; single-unit orders accepted, payment in USD by company transfer, and items can be combined into one shipment.
Browse related products: Rna & dna extraction kits.