CYCF03 is recombinant human keratinocyte growth factor-2 (KGF-2), a member of the fibroblast growth factor family, expressed in E. coli. It is a non-glycosylated polypeptide of 169 amino acids with a molecular weight of 19.2 kDa, supplied as a sterile lyophilized powder.
Catalog no. CYCF03 · For research use only
Key features
- Non-glycosylated 169-amino-acid polypeptide, 19.2 kDa, produced in an E. coli expression system
- Purity above 95.0% by SEC-HPLC and by reducing and non-reducing SDS-PAGE with silver staining
- Bioactivity: ED50 below 2 ng/mL in a dose-dependent BALB/c 3T3 proliferation assay (specific activity 1.0 x 10^5 IU/mg)
- Endotoxin below 0.1 ng/μg (1 EU/μg) by LAL; isoelectric point 9.9 +/- 0.3
- Sterile lyophilized powder, 0.2 μm filtered; for research use only
Catalog numbers and pack sizes
| Catalog # | Product | Pack size |
|---|---|---|
| CYCF00310 | Recombinant Human KGF-2 | 10 μg |
| CYCF00350 | Recombinant Human KGF-2 | 50 μg |
| CYCF003100 | Recombinant Human KGF-2 | 100 μg |
| CYCF003200 | Recombinant Human KGF-2 | 200 μg |
| CYCF003500 | Recombinant Human KGF-2 | 500 μg |
| CYCF0031000 | Recombinant Human KGF-2 | 1 mg |
Specifications
| Source | E. coli, recombinant human KGF-2 |
|---|---|
| Molecular weight | 19.2 kDa (169 amino acids, non-glycosylated) |
| Purity | >95.0% (SEC-HPLC; SDS-PAGE, silver stain) |
| Bioactivity | ED50 <2 ng/mL (BALB/c 3T3), 1.0 x 10^5 IU/mg |
| Endotoxin | <0.1 ng/μg (1 EU/μg), LAL |
| Isoelectric point | 9.9 +/- 0.3 (IEF) |
| Formulation | Lyophilized from 20 mM phosphate, pH 7.4 |
| Storage | Below -18 °C, at least one year (lyophilized) |
| Use | Research use only |
Applications
- Studies of KGF receptor (KGFR) signalling
- Epithelial repair research in skin, stomach, intestine, kidney, bladder and lung models
- Tissue and organ development research
Ordering
Single-unit orders are accepted. Quotes and invoices are in USD with payment by company bank transfer, and different items can be combined into one shipment. Send the catalog number, quantity and destination via the quote form.
Recombinant Human KGF-2 FAQ
What is KGF-2?
KGF-2 belongs to the fibroblast growth factor family and acts through specific binding to the KGF receptor (KGFR). It is described as involved in organ development and epithelial injury repair.
How do I reconstitute it?
Dissolve the lyophilized powder in sterile ultrapure water (18 MΩ-cm) at a concentration of at least 100 μg/mL, then dilute further to the working concentration.
How should CYCF03 be stored?
The lyophilized powder keeps for at least one year below -18 °C and has been tested stable for 3 weeks at room temperature. After reconstitution it is stable for 2-7 days at 4 °C, or at least three months aliquoted below -18 °C. Avoid repeated freeze-thaw cycles.
How do I order CYCF03?
Request a quote with the catalog number; single-unit orders accepted, payment in USD by company transfer, and items can be combined into one shipment.
Browse related products: Research reagents.