CYCF02 is recombinant human basic fibroblast growth factor (bFGF) expressed in E. coli and supplied as a sterile lyophilized powder. It is a non-glycosylated polypeptide with a molecular weight of 16.2 kDa. Sizes range from 10 μg to 1 mg.
Catalog no. CYCF02 · For research use only
Key features
- Non-glycosylated polypeptide, 16.2 kDa, produced in an E. coli expression system
- Purity above 95.0% by reducing and non-reducing SDS-PAGE with silver staining
- Bioactivity: ED50 below 2 ng/mL in a dose-dependent BALB/c 3T3 proliferation assay (specific activity 2.0 x 10^5 IU/mg)
- Endotoxin below 0.1 ng/μg (1 EU/μg) by LAL; isoelectric point 9.6
- Sterile lyophilized powder, 0.2 μm filtered; for research use only
Catalog numbers and pack sizes
| Catalog # | Product | Pack size |
|---|---|---|
| CYCF00210 | Recombinant Human bFGF | 10 μg |
| CYCF00250 | Recombinant Human bFGF | 50 μg |
| CYCF002100 | Recombinant Human bFGF | 100 μg |
| CYCF002200 | Recombinant Human bFGF | 200 μg |
| CYCF002500 | Recombinant Human bFGF | 500 μg |
| CYCF0021000 | Recombinant Human bFGF | 1 mg |
Specifications
| Source | E. coli, recombinant human bFGF |
|---|---|
| Molecular weight | 16.2 kDa (non-glycosylated) |
| Purity | >95.0% (SDS-PAGE, silver stain) |
| Bioactivity | ED50 <2 ng/mL (BALB/c 3T3), 2.0 x 10^5 IU/mg |
| Endotoxin | <0.1 ng/μg (1 EU/μg), LAL |
| Isoelectric point | 9.6 (IEF) |
| Formulation | Lyophilized from 20 mM phosphate, 200 mM NaCl, pH 7.4 |
| Storage | Below -18 °C, at least one year (lyophilized) |
| Use | Research use only |
Applications
- Growth stimulation of cells derived from mesoderm and neuroectoderm in culture
- Studies of fibroblast, endothelial, epithelial, neural, muscle and bone cell growth
- Growth-factor signalling research
Ordering
Single-unit orders are accepted. Quotes and invoices are in USD with payment by company bank transfer, and different items can be combined into one shipment. Send the catalog number, quantity and destination via the quote form.
Recombinant Human bFGF FAQ
Which cell types respond to bFGF?
bFGF stimulates growth of cells of mesodermal and neuroectodermal origin, such as fibroblasts, vascular endothelial cells, corneal cells, epithelial cells, neural cells, muscle cells and bone cells.
How do I reconstitute it?
Dissolve the lyophilized powder in sterile ultrapure water (18 MΩ-cm) at a concentration of at least 100 μg/mL, then dilute further to the working concentration.
How should CYCF02 be stored?
The lyophilized powder keeps for at least one year below -18 °C and has been tested stable for 3 weeks at room temperature. After reconstitution it is stable for 2-7 days at 4 °C, or at least three months aliquoted below -18 °C. Avoid repeated freeze-thaw cycles.
How do I order CYCF02?
Request a quote with the catalog number; single-unit orders accepted, payment in USD by company transfer, and items can be combined into one shipment.
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