Changyu Bio quotes QIAGEN catalog number 150343 (REPLI-g Single Cell Kit, 24 reactions) for research laboratories worldwide, including buyers in Japan, Southeast Asia, Africa and Europe. Brand names identify the catalog item only. No price is published. Research Use Only.
Specifications
| Catalog number | 150343 (24 reactions) |
| Product | REPLI-g Single Cell Kit |
| Kit contents | REPLI-g sc Polymerase, REPLI-g sc Reaction Buffer (salt, primers, dNTPs), Buffer DLB (lysis and denaturation) |
| Sample input | Single cells (1 to <1000 bacterial or tumor cells) or purified genomic DNA |
| Technology | Multiple Displacement Amplification (MDA), isothermal, Phi29 polymerase; not PCR-based |
| Typical yield | Up to 40 µg DNA per reaction |
| Product length | Average >10 kb |
| Hands-on time | About 15 minutes |
| Downstream use | NGS (whole genome / exome), SNP arrays, array CGH, qPCR / PCR, STR, Sanger sequencing |
| Intended use | Molecular biology research. Not for diagnosis, prevention or treatment of disease. |
What this kit is for
Whole genome amplification (WGA) from single cells or very small amounts of genomic DNA, giving enough DNA for sequencing and genotyping. Cells are lysed and DNA denatured with a gentle alkaline step, then amplified at constant temperature by Phi29 polymerase with random primers.
Request a quote
Send the catalog number (150343), quantity and destination city. Availability, lot and lead time are confirmed on each quotation. Shipping conditions follow QIAGEN’s storage requirements.
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QIAGEN and REPLI-g are trademarks of QIAGEN. This page is for catalog matching and research quotation only. Specifications follow QIAGEN’s public product page; re-check the current catalog before ordering. All products RUO.
Common questions
- What is QIAGEN 150343? REPLI-g Single Cell Kit, 24 whole genome amplification reactions.
- What input does it accept? Single cells (1 to fewer than 1000 bacterial or tumor cells) or purified genomic DNA.
- How much DNA does one reaction give? QIAGEN states yields of up to 40 µg per reaction, with average product length over 10 kb.
- Is REPLI-g a PCR method? No. It uses isothermal Multiple Displacement Amplification with Phi29 polymerase.
- Can you quote 150343 for Japan? Yes. Send the quantity and destination city; availability and lead time are confirmed on the quotation.