CYDN54 is a DNA bisulfite conversion kit for DNA methylation studies. Bisulfite converts unmethylated cytosine to uracil while methylated cytosine is unchanged. The kit covers denaturation, sulfonation, deamination and desulfonation with column purification.
Catalog no. CYDN54 · For research use only
Key features
- Conversion of unmethylated cytosine to uracil above 99%
- Input from 500 pg to 2.5 μg of DNA
- DNA protectant reduces fragmentation and improves recovery; compatible with tissue, cells, whole blood and FFPE samples
- Ultra-micro washerless spin column with minimum elution of 5 μL; on-column desulfonation
- Converted DNA suited to MSP, BSP, pyrosequencing, qPCR and high-throughput sequencing; research use only
Catalog numbers and pack sizes
| Catalog # | Product | Pack size |
|---|---|---|
| CYDN5401 | DNA Bisulfite Conversion Kit | 50 preps |
| CYDN5402 | DNA Bisulfite Conversion Kit | 100 preps |
Specifications
| Format | Spin column, silica membrane |
|---|---|
| Conversion | Over 99% |
| Input | 500 pg to 2.5 μg |
| Min. elution volume | 5 μL |
| Sample | Tissue, cells, whole blood, FFPE |
| Use | Research use only |
Applications
- DNA methylation analysis (MSP, BSP)
- Pyrosequencing
- qPCR
- High-throughput sequencing
Ordering
Single-unit orders are accepted. Quotes and invoices are in USD with payment by company bank transfer, and different items can be combined into one shipment. Send the catalog number, quantity and destination via the quote form.
DNA Bisulfite Conversion Kit FAQ
What is the conversion rate?
Over 99% of unmethylated cytosine is converted to uracil.
What DNA input is supported?
From 500 pg to 2.5 μg.
Which sample types are compatible?
Tissue, cells, whole blood and FFPE samples.
How do I order CYDN54?
Request a quote with the catalog number; single-unit orders accepted, payment in USD by company transfer, and items can be combined into one shipment.
Browse related products: Rna & dna extraction kits.