CYDN21 is a spin-column kit for purifying single-stranded phage DNA from the supernatant of cultures infected with M13 filamentous phage or M13-derived phagemids. M13 vectors supply single-stranded DNA for library construction, sequencing and mutagenesis.
Catalog no. CYDN21 · For research use only
Key features
- Typical yield 3 μg of single-stranded DNA from 800 μL phage supernatant
- A single sample usually completed within 10 minutes
- No toxic phenol and no ethanol precipitation
- Typical OD260/OD280 of 1.7-1.9
- DNA can be sequenced directly, with typical read lengths up to about 650 bp; research use only
Catalog numbers and pack sizes
| Catalog # | Product | Pack size |
|---|---|---|
| CYDN2101 | M13 Phage ssDNA Kit | 50 preps |
| CYDN2102 | M13 Phage ssDNA Kit | 100 preps |
| CYDN2103 | M13 Phage ssDNA Kit | 200 preps |
Specifications
| Format | Spin column, silica membrane |
|---|---|
| Sample | M13 phage or phagemid culture supernatant |
| Yield | About 3 μg per 800 μL supernatant |
| Time | About 10 minutes |
| Typical OD260/OD280 | 1.7-1.9 |
| Use | Research use only |
Applications
- Sequencing template preparation
- Library construction
- Mutagenesis work
Ordering
Single-unit orders are accepted. Quotes and invoices are in USD with payment by company bank transfer, and different items can be combined into one shipment. Send the catalog number, quantity and destination via the quote form.
M13 Phage ssDNA Kit FAQ
What yield is typical?
About 3 μg of single-stranded DNA from 800 μL M13 phage supernatant.
How long does it take?
Generally within 10 minutes.
Can the DNA be sequenced directly?
Yes, with typical readable lengths up to about 650 bp.
How do I order CYDN21?
Request a quote with the catalog number; single-unit orders accepted, payment in USD by company transfer, and items can be combined into one shipment.
Browse related products: Rna & dna extraction kits.