CYDN17 is a kit for selectively isolating the apoptotic DNA ladder (fragments of about n x 185 bp) from cells and tissue, separating it from intact genomic DNA to reduce interference and raise detection sensitivity. The reaction runs in microcentrifuge tubes and takes about 2.5 hours, without organic extraction.
Catalog no. CYDN17 · For research use only
Key features
- Selective separation of genomic DNA from the DNA ladder improves sensitivity
- A DNA ladder can be detected from about 2,000 apoptotic cells
- Recommended input 5-10 x 10^5 cells, adjustable between 1 x 10^5 and 5 x 10^6, with at least about 1-2 x 10^4 apoptotic cells in the sample
- More than 2 x 10^4 apoptotic cells usually gives a clear ladder
- Enzyme B arrives lyophilized: dissolve in 500 μL sterile water (25 preps) or 1 mL (50 preps) and store at -20 °C; avoid repeated freeze-thaw of Enzyme A and B; research use only
Catalog numbers and pack sizes
| Catalog # | Product | Pack size |
|---|---|---|
| CYDN1701 | Selective Apoptotic DNA Ladder Extraction Kit | 25 preps |
| CYDN1702 | Selective Apoptotic DNA Ladder Extraction Kit | 50 preps |
Specifications
| Format | Solution-based, microcentrifuge tubes |
|---|---|
| Sample | Cultured cells; tissue also possible |
| Time | About 2.5 hours |
| Storage | Enzymes at -20 °C, aliquoted to avoid freeze-thaw |
| Use | Research use only |
Applications
- Apoptosis research
- Detection of apoptotic DNA ladder by agarose gel electrophoresis
Ordering
Single-unit orders are accepted. Quotes and invoices are in USD with payment by company bank transfer, and different items can be combined into one shipment. Send the catalog number, quantity and destination via the quote form.
Selective Apoptotic DNA Ladder Extraction Kit FAQ
How sensitive is it?
A DNA ladder can be detected from about 2,000 apoptotic cells.
How should Enzyme B be prepared?
Dissolve the lyophilized powder in 500 μL (25 preps) or 1 mL (50 preps) sterile water and store at -20 °C.
Can tissue be used?
Yes, but timing and extent of apoptosis vary in whole-animal tissue, so sampling affects results.
How do I order CYDN17?
Request a quote with the catalog number; single-unit orders accepted, payment in USD by company transfer, and items can be combined into one shipment.
Browse related products: Rna & dna extraction kits.