CYRN45 is an RNase-free DNase I kit (1500 units) for digesting DNA. DNase I is an endonuclease that digests single- or double-stranded DNA into mono- or oligodeoxynucleotides with 5′ phosphate and 3′ hydroxyl ends.
Catalog no. CYRN45 · For research use only
Key features
- Activity is calcium-dependent and activated by magnesium or manganese ions
- With magnesium, DNase I cuts double-stranded DNA at random sites; with manganese it cuts both strands at the same site
- The reaction buffer allows use without phenol/chloroform extraction inactivation, so samples can go directly to reverse transcription
- Fast and convenient to use
- Research use only
Catalog numbers and pack sizes
| Catalog # | Product | Pack size |
|---|---|---|
| CYRN4501 | DNase I Rapid DNA Digestion Kit (RNase-Free) | 1500 U |
Specifications
| Enzyme | DNase I, RNase-free |
|---|---|
| Amount | 1500 units |
| Activators | Mg2+ or Mn2+; Ca2+ dependent |
| Product ends | 5′ phosphate, 3′ hydroxyl |
| Use | Research use only |
Applications
- Removal of DNA from RNA samples
- Pre-reverse-transcription DNA digestion
Ordering
Single-unit orders are accepted. Quotes and invoices are in USD with payment by company bank transfer, and different items can be combined into one shipment. Send the catalog number, quantity and destination via the quote form.
DNase I Rapid DNA Digestion Kit (RNase-Free) FAQ
What products does DNase I make?
Mono- or oligodeoxynucleotides with 5′ phosphate and 3′ hydroxyl ends.
Is phenol/chloroform inactivation needed?
No, the reaction can go directly into reverse transcription.
What ions does it need?
Calcium dependence, activated by magnesium or manganese.
How do I order CYRN45?
Request a quote with the catalog number; single-unit orders accepted, payment in USD by company transfer, and items can be combined into one shipment.
Browse related products: Rna & dna extraction kits.