CYPL10 is a spin-column endotoxin-free plasmid kit for transfection-grade DNA. A modified SDS-alkaline lysis is followed by a special endotoxin removal agent and silica membrane binding.
Catalog no. CYPL10 · For research use only
Key features
- Endotoxin removal reported generally below 0.1 EU/μg
- 30-90 μg high-copy plasmid from 5-15 mL LB culture, over 80% recovery
- Protein removal solution clears nucleases, including from JM series and HB101
- No toxic phenol or chloroform and no ethanol precipitation
- Usable for transfection, digestion, transformation, PCR, in vitro transcription and sequencing; research use only
Catalog numbers and pack sizes
| Catalog # | Product | Pack size |
|---|---|---|
| CYPL1001 | EndoFree Plasmid Midi Kit | 50 preps |
| CYPL1002 | EndoFree Plasmid Midi Kit | 100 preps |
Specifications
| Format | Spin column, silica membrane |
|---|---|
| Endotoxin | Generally below 0.1 EU/μg |
| Yield | 30-90 μg from 5-15 mL LB culture |
| Recovery | Over 80% |
| Use | Research use only |
Applications
- Eukaryotic cell transfection
- Digestion and PCR
- Transformation
- In vitro transcription and sequencing
Ordering
Single-unit orders are accepted. Quotes and invoices are in USD with payment by company bank transfer, and different items can be combined into one shipment. Send the catalog number, quantity and destination via the quote form.
EndoFree Plasmid Midi Kit FAQ
What endotoxin level is reported?
Generally below 0.1 EU/μg.
What yield is typical?
30-90 μg of high-copy plasmid from 5-15 mL LB culture.
Does it use phenol?
No, nor chloroform or ethanol precipitation.
How do I order CYPL10?
Request a quote with the catalog number; single-unit orders accepted, payment in USD by company transfer, and items can be combined into one shipment.
Browse related products: Rna & dna extraction kits.