CYPL19 is a large-scale plasmid purification kit for yeast. It combines modified SDS-alkaline lysis with lytic enzyme digestion of the cell wall, giving plasmid DNA in about 1 hour.
Catalog no. CYPL19 · For research use only
Key features
- Lytic enzyme removes the yeast cell wall before alkaline lysis
- Plasmid DNA binds silica membrane at high salt and low pH
- No toxic phenol or chloroform and no ethanol precipitation
- High yield and purity; DNA usable for digestion, transformation, PCR, in vitro transcription and sequencing
- Research use only
Catalog numbers and pack sizes
| Catalog # | Product | Pack size |
|---|---|---|
| CYPL1901 | Yeast Plasmid Maxi Kit | 10 preps |
Specifications
| Format | Spin column, silica membrane |
|---|---|
| Sample | Yeast culture |
| Time | About 1 hour |
| Scale | Maxi |
| Use | Research use only |
Applications
- Yeast plasmid isolation
- Restriction digestion
- Transformation
- PCR and sequencing
Ordering
Single-unit orders are accepted. Quotes and invoices are in USD with payment by company bank transfer, and different items can be combined into one shipment. Send the catalog number, quantity and destination via the quote form.
Yeast Plasmid Maxi Kit FAQ
How is the cell wall removed?
With a lytic enzyme before alkaline lysis.
How long does it take?
About 1 hour.
Is phenol needed?
No.
How do I order CYPL19?
Request a quote with the catalog number; single-unit orders accepted, payment in USD by company transfer, and items can be combined into one shipment.
Browse related products: Rna & dna extraction kits.