CYPL06 is a spin-column plasmid miniprep kit for yeast. It combines a modified SDS-alkaline lysis with lytic enzyme digestion of the yeast cell wall and delivers plasmid DNA in about 1 hour.
Catalog no. CYPL06 · For research use only
Key features
- Lytic enzyme (lyticase) digests the yeast cell wall before alkaline lysis
- Plasmid DNA binds a silica membrane at high salt and low pH
- No toxic phenol or chloroform and no ethanol precipitation
- Plasmid usable for digestion, transformation, PCR, in vitro transcription and sequencing
- Research use only
Catalog numbers and pack sizes
| Catalog # | Product | Pack size |
|---|---|---|
| CYPL0601 | Yeast Plasmid Mini Kit | 50 preps |
Specifications
| Format | Spin column, silica membrane |
|---|---|
| Sample | Yeast culture |
| Method | Lyticase digestion + SDS-alkaline lysis |
| Time | About 1 hour |
| Use | Research use only |
Applications
- Yeast plasmid isolation
- Restriction digestion
- Transformation
- PCR and sequencing
Ordering
Single-unit orders are accepted. Quotes and invoices are in USD with payment by company bank transfer, and different items can be combined into one shipment. Send the catalog number, quantity and destination via the quote form.
Yeast Plasmid Mini Kit FAQ
How does it handle the yeast cell wall?
Lytic enzyme digests the cell wall before alkaline lysis.
How long does it take?
About 1 hour.
Is phenol needed?
No, nor chloroform or ethanol precipitation.
How do I order CYPL06?
Request a quote with the catalog number; single-unit orders accepted, payment in USD by company transfer, and items can be combined into one shipment.
Browse related products: Rna & dna extraction kits.