CYPL04 is a spin-column endotoxin-free plasmid miniprep kit for use in eukaryotic cell transfection. It combines high-yield alkaline lysis, silica membrane binding and a special endotoxin removal agent.
Catalog no. CYPL04 · For research use only
Key features
- Endotoxin removal reported generally below 0.1 EU/μg
- 20-50 μg of high-copy plasmid from 5 mL LB culture, over 80% recovery
- High-yield lysis raises yield 1-2 times
- Protein removal solution clears nucleases, including from JM series and HB101
- Suited to protoplast and eukaryotic cell transfection; research use only
Catalog numbers and pack sizes
| Catalog # | Product | Pack size |
|---|---|---|
| CYPL0401 | EndoFree Plasmid Mini Kit | 50 preps |
| CYPL0402 | EndoFree Plasmid Mini Kit | 100 preps |
Specifications
| Format | Spin column, silica membrane |
|---|---|
| Endotoxin | Generally below 0.1 EU/μg |
| Yield | 20-50 μg from 5 mL LB culture (high-copy plasmid) |
| Recovery | Over 80% |
| Use | Research use only |
Applications
- Eukaryotic cell transfection
- Protoplast transfection
- Digestion and PCR
- Sequencing
Ordering
Single-unit orders are accepted. Quotes and invoices are in USD with payment by company bank transfer, and different items can be combined into one shipment. Send the catalog number, quantity and destination via the quote form.
EndoFree Plasmid Mini Kit FAQ
What endotoxin level is reported?
Generally below 0.1 EU/μg.
What yield is typical?
20-50 μg of high-copy plasmid from 5 mL LB culture.
Can it be used for transfection?
Yes, including protoplast and eukaryotic cell transfection.
How do I order CYPL04?
Request a quote with the catalog number; single-unit orders accepted, payment in USD by company transfer, and items can be combined into one shipment.
Browse related products: Rna & dna extraction kits.