CYPL03 is a spin-column plasmid miniprep kit using a modified SDS-alkaline lysis. Its protein removal solution efficiently removes residual nucleases, including from nuclease-rich strains such as the JM series and HB101.
Catalog no. CYPL03 · For research use only
Key features
- Modified SDS-alkaline lysis; plasmid DNA binds silica membrane in high salt, low pH
- Protein removal solution removes nucleases, helping prevent plasmid degradation
- Works on nuclease-rich strains such as JM series and HB101
- No toxic phenol or chloroform and no ethanol precipitation
- Plasmid usable for digestion, transformation, PCR, in vitro transcription and sequencing; research use only
Catalog numbers and pack sizes
| Catalog # | Product | Pack size |
|---|---|---|
| CYPL0301 | High Pure Plasmid Mini Kit | 50 preps |
| CYPL0302 | High Pure Plasmid Mini Kit | 100 preps |
| CYPL0303 | High Pure Plasmid Mini Kit | 200 preps |
Specifications
| Format | Spin column, silica membrane |
|---|---|
| Method | Modified SDS-alkaline lysis |
| Strains | Including nuclease-rich JM series and HB101 |
| Elution | Low-salt, high-pH buffer |
| Use | Research use only |
Applications
- Restriction digestion
- Transformation
- PCR
- In vitro transcription
- Sequencing
Ordering
Single-unit orders are accepted. Quotes and invoices are in USD with payment by company bank transfer, and different items can be combined into one shipment. Send the catalog number, quantity and destination via the quote form.
High Pure Plasmid Mini Kit FAQ
Which strains is it suited to?
Including nuclease-rich strains such as JM series and HB101.
How are nucleases controlled?
A special protein removal solution removes residual nucleases to protect the plasmid.
Is phenol needed?
No, nor chloroform or ethanol precipitation.
How do I order CYPL03?
Request a quote with the catalog number; single-unit orders accepted, payment in USD by company transfer, and items can be combined into one shipment.
Browse related products: Rna & dna extraction kits.