CYRN01 is a total RNA isolation reagent for cells and tissues of human, animal, plant and bacterial origin. After chloroform addition and centrifugation, RNA stays in the aqueous phase while DNA and protein can be recovered from the interphase and organic phase; the workflow is comparable to TRIzol.
Catalog no. CYRN01 · For research use only
Key features
- Maintains RNA integrity during cell disruption and lysis
- Works for small samples (50-100 mg tissue, 5 x 10^6 cells) and large samples (1 g or more, over 10^7 cells)
- All steps can be done within one hour; multiple samples handled together
- RNA free of DNA and protein contamination; A260/A280 of 1.8 or higher in TE
- Co-purified DNA helps normalize RNA yield between samples
- Research use only
Catalog numbers and pack sizes
| Catalog # | Product | Pack size |
|---|---|---|
| CYRN0101 | TRIpure Reagent | 50 mL |
| CYRN0102 | TRIpure Reagent | 100 mL |
Specifications
| Format | Liquid reagent |
|---|---|
| Sample | Animal and plant tissue, cells, bacteria |
| Co-isolation | Total RNA, DNA, protein |
| RNA purity | A260/A280 of 1.8 or higher in TE |
| Use | Research use only |
Applications
- RNA blotting
- Dot blot
- Poly(A)+ selection
- In vitro translation
- RNase protection assay and molecular cloning
Ordering
Single-unit orders are accepted. Quotes and invoices are in USD with payment by company bank transfer, and different items can be combined into one shipment. Send the catalog number, quantity and destination via the quote form.
TRIpure Reagent FAQ
Which phase contains the RNA?
The upper aqueous phase after chloroform addition and centrifugation; RNA is recovered by isopropanol precipitation.
Can DNA and protein be recovered?
Yes, DNA by ethanol precipitation from the interphase and protein by isopropanol from the organic phase.
Is DNase treatment needed for PCR?
If both primers lie within a single exon, treating the RNA with amplification-grade DNase I is recommended.
How do I order CYRN01?
Request a quote with the catalog number; single-unit orders accepted, payment in USD by company transfer, and items can be combined into one shipment.
Browse related products: Rna & dna extraction kits.