CYPC45 is a one-step RT-PCR kit that runs reverse transcription and PCR in one tube using a thermostable reverse transcriptase. It includes an OneStep Enzyme Mix and a 2x RT-PCR Mix.
Catalog no. CYPC45 · For research use only
Key features
- OneStep Enzyme Mix: thermostable THERMOscript M-MuLV H Minus RTase, hot-start HotMaster Taq and RNasin inhibitor
- 2x RT-PCR Mix suited to both RT and PCR
- Reverse transcription at 50-55 °C improves efficiency on GC-rich, structured templates
- No lid opening between RT and PCR; limits contamination
- RNase H-minus enzyme with stronger extension ability than wild-type; research use only
Catalog numbers and pack sizes
| Catalog # | Product | Pack size |
|---|---|---|
| CYPC4501 | THERMOscript One Step RT-PCR Kit | 100 reactions x 20 μL |
| CYPC4502 | THERMOscript One Step RT-PCR Kit | 200 reactions x 20 μL |
Specifications
| Format | One-step RT-PCR kit |
|---|---|
| Reaction size | 20 μL |
| RT temperature | 50-55 °C |
| Components | OneStep Enzyme Mix, 2x RT-PCR Mix |
| Storage | -20 °C, 6 months |
| Use | Research use only |
Applications
- One-step RT-PCR
- Amplification from GC-rich or structured RNA
Ordering
Single-unit orders are accepted. Quotes and invoices are in USD with payment by company bank transfer, and different items can be combined into one shipment. Send the catalog number, quantity and destination via the quote form.
THERMOscript One Step RT-PCR Kit FAQ
What RT temperature is used?
50-55 °C.
What does the enzyme mix contain?
A thermostable M-MuLV H Minus reverse transcriptase, hot-start HotMaster Taq DNA polymerase and an RNasin inhibitor mix.
What is the shelf life?
6 months at -20 °C.
How do I order CYPC45?
Request a quote with the catalog number; single-unit orders accepted, payment in USD by company transfer, and items can be combined into one shipment.
Browse related products: Pcr & qpcr master mixes.