CYPC64 is a high-temperature reverse transcription kit with genomic DNA removal for real-time RT-PCR. Its enzyme works at up to 60 °C and reads through GC-rich RNA with complex secondary structure.
Catalog no. CYPC64 · For research use only
Key features
- gDNA Eraser Mix removes residual genomic DNA in 2 minutes, no DNase digestion
- Premixed 5x THERMO RT MasterMix II: RTase, RNase inhibitor, random primer, Oligo dT primer, dNTP mixture, buffer
- Reverse transcription in 15 minutes after adding RNA and water
- Ct values are reported to come 2-3 cycles earlier in general
- Oligo dT and N6 ratio optimized for qPCR
- Research use only
Catalog numbers and pack sizes
| Catalog # | Product | Pack size |
|---|---|---|
| CYPC6401 | THERMOscript RT Kit (+gDNA Eraser) | 50 reactions |
| CYPC6402 | THERMOscript RT Kit (+gDNA Eraser) | 100 reactions |
Specifications
| Format | Two-step kit: gDNA Eraser Mix + THERMO RT MasterMix II |
|---|---|
| Temperature | Up to 60 °C |
| gDNA removal | 2 minutes |
| RT time | 15 minutes |
| Storage | -20 °C, 12 months |
| Use | Research use only |
Applications
- cDNA synthesis for real-time RT-PCR
- Reverse transcription of GC-rich or structured RNA
Ordering
Single-unit orders are accepted. Quotes and invoices are in USD with payment by company bank transfer, and different items can be combined into one shipment. Send the catalog number, quantity and destination via the quote form.
THERMOscript RT Kit (+gDNA Eraser) FAQ
What is the reaction temperature?
The enzyme is described for use at up to 60 °C.
Is DNase I digestion required?
No. The 4x gDNA Eraser Mix removes genomic DNA in 2 minutes.
How is it stored?
At -20 °C, with a 12-month shelf life.
How do I order CYPC64?
Request a quote with the catalog number; single-unit orders accepted, payment in USD by company transfer, and items can be combined into one shipment.
Browse related products: Pcr & qpcr master mixes.