CYPC54 is a reverse transcription kit that removes genomic DNA contamination and then synthesizes first-strand cDNA for real-time RT-PCR. A 4x gDNA Eraser Mix removes residual gDNA in 2 minutes, followed by a premixed 5x RT MasterMix II.
Catalog no. CYPC54 · For research use only
Key features
- gDNA Eraser removes DNA residue in 2 minutes, with no DNase digestion step
- Premixed RT MasterMix II holds RTase, RNase inhibitor, random primers, Oligo dT primer, dNTP mixture and buffer
- Add only RNA and water; reverse transcription takes 15 minutes
- Both mixes resist freezing at -20 °C
- Oligo dT to N6 random primer ratio optimized for qPCR
- Research use only
Catalog numbers and pack sizes
| Catalog # | Product | Pack size |
|---|---|---|
| CYPC5401 | TRUEscript RT Kit (+gDNA Eraser) | 50 reactions |
| CYPC5402 | TRUEscript RT Kit (+gDNA Eraser) | 100 reactions |
Specifications
| Format | Two-step kit: 4x gDNA Eraser Mix + 5x TRUE RT MasterMix II |
|---|---|
| gDNA removal | 2 minutes |
| RT time | 15 minutes |
| Primers | Oligo dT and random primers included |
| Storage | -20 °C, 12 months |
| Use | Research use only |
Applications
- cDNA synthesis for real-time RT-PCR
- Reverse transcription with genomic DNA removal
Ordering
Single-unit orders are accepted. Quotes and invoices are in USD with payment by company bank transfer, and different items can be combined into one shipment. Send the catalog number, quantity and destination via the quote form.
TRUEscript RT Kit (+gDNA Eraser) FAQ
How is genomic DNA removed?
A 4x gDNA Eraser Mix with DNA-degrading activity removes residual gDNA in 2 minutes, so DNase I digestion is not needed.
How long does reverse transcription take?
About 15 minutes after adding RNA and water to the premix.
Why is the primer ratio mentioned?
The Oligo dT and N6 random primer ratio is optimized for qPCR so cDNA synthesis can start from all regions of the transcript.
How do I order CYPC54?
Request a quote with the catalog number; single-unit orders accepted, payment in USD by company transfer, and items can be combined into one shipment.
Browse related products: Pcr & qpcr master mixes.