CYPC43 is a thermostable RNase H-minus M-MuLV reverse transcriptase for first-strand cDNA synthesis at elevated temperature. Multi-point mutations allow reverse transcription at 55-60 °C, with partial activity loss at higher temperatures.
Catalog no. CYPC43 · For research use only
Key features
- RNase H-minus recombinant M-MuLV reverse transcriptase with improved temperature tolerance
- Reverse transcription at 55-60 °C; activity partly decreases above this range
- Higher reaction temperature improves transcription of GC-rich RNA with abundant secondary structure
- Stronger extension ability and stability than wild-type M-MuLV
- 200 units/μL; for research use only.
Catalog numbers and pack sizes
| Catalog # | Product | Pack size |
|---|---|---|
| CYPC4302 | THERMOscript H Minus M-MuLV Reverse Transcriptase | 10,000 U |
| CYPC4303 | THERMOscript H Minus M-MuLV Reverse Transcriptase | 10,000 U x 5 tubes |
Specifications
| Enzyme | THERMOscript M-MuLV (RNase H-minus) |
|---|---|
| Concentration | 200 units/μL |
| Reaction temperature | 55-60 °C |
| Storage | -20 °C |
| Use | Research use only |
Applications
- cDNA synthesis from GC-rich RNA
- Transcription of RNA with strong secondary structure
- Long cDNA synthesis
- Full-length cDNA library construction
Ordering
Single-unit orders are accepted. Quotes and invoices are in USD with payment by company bank transfer, and different items can be combined into one shipment. Send the catalog number, quantity and destination via the quote form.
THERMOscript H Minus M-MuLV Reverse Transcriptase FAQ
What temperature range can it be used at?
Reverse transcription can be run at up to 55-60 °C; activity is partly reduced at higher temperatures.
Why use a high-temperature enzyme?
The higher temperature improves reverse transcription efficiency on GC-rich templates with abundant secondary structure.
How should it be stored?
Store at -20 °C; concentration is 200 units/μL.
How do I order CYPC43?
Request a quote with the catalog number; single-unit orders accepted, payment in USD by company transfer, and items can be combined into one shipment.
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