CYEP11 is a red-fluorescent, GelRed-type nucleic acid gel stain, 10,000x in DMSO, designed to replace ethidium bromide (EB). Because its spectral properties match EB, it can be used without changing the imaging system or filters. It is applied pre-cast at 10,000-fold dilution or post-stain at about 3,300-fold dilution.
Catalog no. CYEP11 · For research use only
Key features
- Large, oily dye molecule that does not cross cell membranes; Ames test results indicate much lower mutagenicity than EB
- High sensitivity for fragments of all sizes with little effect on nucleic acid migration
- Stable in heated gel preparation (microwave), in acid or alkaline buffers at room temperature, and under light
- Pre-cast (1:10,000) or post-stain (about 1:3,300) for dsDNA, ssDNA or RNA; same spectral properties as EB
- Standard EB or SYBR filters work; best excitation near 300 nm UV
- For research use only
Catalog numbers and pack sizes
| Catalog # | Product | Pack size |
|---|---|---|
| CYEP1101 | Red Nucleic Acid Gel Stain (GelRed-Type, 10,000x) | 500 μL (10,000x) |
Specifications
| Format | 10,000x concentrate in DMSO |
|---|---|
| Nucleic acids | dsDNA, ssDNA, RNA |
| Gels | Agarose or polyacrylamide |
| Staining | Pre-cast (10,000x dilution) or post-stain (about 3,300x) |
| Volume | 500 μL |
| Excitation | Same spectral properties as EB; standard EB or SYBR filters and a UV transilluminator near 300 nm give best excitation. Not fully excited by 488 nm argon-ion lasers or similar visible light, so a green-fluorescent stain such as CYEP10 is advised there |
| Use | Research use only |
Applications
- Agarose gel staining of DNA and RNA
- Replacing EB with a lower-mutagenicity stain
- Gel imaging on a standard UV transilluminator
Ordering
Single-unit orders are accepted. Quotes and invoices are in USD with payment by company bank transfer, and different items can be combined into one shipment. Send the catalog number, quantity and destination via the quote form.
Red Nucleic Acid Gel Stain (GelRed-Type, 10,000x) FAQ
Pre-cast or post-stain?
Both work. Post-staining is usually more sensitive and avoids any effect on band separation; pre-cast is simpler and cheaper because it needs no staining step and uses less dye. Trying both is suggested.
Which instruments suit it?
Same spectral properties as EB; standard EB or SYBR filters and a UV transilluminator near 300 nm give best excitation. Not fully excited by 488 nm argon-ion lasers or similar visible light, so a green-fluorescent stain such as CYEP10 is advised there
Is it safe?
It is designed with a large oily molecule that cannot penetrate cell membranes, and Ames test results indicate much lower mutagenicity than EB. Standard lab precautions (gloves, proper waste disposal) still apply.
How do I order CYEP11?
Request a quote with the catalog number; single-unit orders accepted, payment in USD by company transfer, and items can be combined into one shipment.
Browse related products: Research reagents.