CYCC08 is a kit for preparing high-efficiency competent E. coli, based on an improved traditional method. Competent efficiency can reach 10^8 cfu/μg plasmid (a little higher for small plasmids, lower for large ones). Once the culture is ready, preparation takes about 60 minutes.
Catalog no. CYCC08 · For research use only
Key features
- Efficiency up to 10^8 cfu/μg plasmid
- About 60 minutes to prepare competent cells from a ready culture
- Suited to plasmids and also to ordinary ligation products, especially blunt-end ligation that needs high efficiency
- Works with most common E. coli strains, including TOP10, DH5α, BL21(DE3), JM109, TG1, HB101 and XL-1; efficiency varies by strain
- Can be used in several batches; cells go to -70 °C quickly; for research use only
Catalog numbers and pack sizes
| Catalog # | Product | Pack size |
|---|---|---|
| CYCC0801 | High-Efficiency Competent Cell Preparation Kit | 100 x 100 μL |
| CYCC0802 | High-Efficiency Competent Cell Preparation Kit | 200 x 100 μL |
| CYCC0803 | High-Efficiency Competent Cell Preparation Kit | 400 x 100 μL |
Specifications
| Format | Competent cell preparation kit |
|---|---|
| Efficiency | Up to 10^8 cfu/μg plasmid |
| Preparation time | About 60 minutes |
| Strains | TOP10, DH5α, BL21(DE3), JM109, TG1, HB101, XL-1 and others |
| Output | 100 tubes of 100 μL (CYCC0801) |
| Storage of cells | -70 °C |
| Use | Research use only |
Applications
- Preparing competent cells in the lab
- Transforming ligation products
- Blunt-end ligation transformation
Ordering
Single-unit orders are accepted. Quotes and invoices are in USD with payment by company bank transfer, and different items can be combined into one shipment. Send the catalog number, quantity and destination via the quote form.
High-Efficiency Competent Cell Preparation Kit FAQ
What efficiency can I expect?
Up to 10^8 cfu/μg plasmid, slightly higher for small plasmids and lower for large ones. Strains differ.
Which strains work?
Most common E. coli strains, including TOP10, DH5α, BL21(DE3), JM109, TG1, HB101 and XL-1.
Any key precautions?
Sterilize all reagents, consumables and equipment. Use antibiotic-free LB throughout preparation and in the 37 °C recovery after heat shock, even for resistant plasmids. Move cells to -70 °C quickly.
How do I order CYCC08?
Request a quote with the catalog number; single-unit orders accepted, payment in USD by company transfer, and items can be combined into one shipment.
Browse related products: Ptopo cloning kits.