CYCV06 is a kit that adds an A tail to blunt-end DNA so it can be ligated into T vectors. PCR products from proofreading polymerases such as Pfu, Pwo, Vent or KOD are blunt and lack the 3′ A needed to pair with a T vector; Taq DNA polymerase adds it. The kit supplies every component for A-tailing, which takes about 20 minutes.
Catalog no. CYCV06 · For research use only
Key features
- Provides all components needed for A-tailing
- For blunt PCR products from Pfu, Pwo, Vent or KOD-type proofreading polymerases
- Whole procedure takes about 20 minutes
- Prepares blunt products for ligation into T vectors
- Stored at -20 °C; for research use only
Catalog numbers and pack sizes
| Catalog # | Product | Pack size |
|---|---|---|
| CYCV0601 | Blunt-End DNA A-Tailing Kit | 25 reactions |
| CYCV0602 | Blunt-End DNA A-Tailing Kit | 50 reactions |
Specifications
| Format | A-tailing kit |
|---|---|
| Input | Blunt-end DNA from proofreading polymerases |
| Time | About 20 minutes |
| Purpose | Adds 3′ A for T-vector ligation |
| Storage | -20 °C |
| Use | Research use only |
Applications
- Adding A tails to blunt PCR products
- Preparing proofreading-enzyme products for T-vector cloning
- TA cloning workflows
Ordering
International ordering — CYCV06
- Single-unit orders are accepted, subject to confirmation — availability and lead time are confirmed on the quotation.
- Different Changyu Bio and branded items can be combined into one shipment.
- International delivery, including Europe, the United States and Canada, Brazil and Latin America, the Middle East, Africa and Asia; destination and import requirements are confirmed before shipment, and air freight typically takes 7–14 days after dispatch.
- Storage: -20 °C. Shipping temperature is confirmed per item.
- Payment by bank transfer (T/T) in US dollars after you confirm the quotation.
- Request a quotation for CYCV06 — tell us the quantity and destination city.
Blunt-End DNA A-Tailing Kit FAQ
Why is A-tailing needed?
Blunt products from proofreading polymerases lack the 3′ A that pairs with the T overhang of a T vector, so A is added with Taq DNA polymerase first.
Which polymerase products does it suit?
Blunt products from Pfu, Pwo, Vent, KOD and other polymerases with 3′-5′ exonuclease activity.
How long does it take?
About 20 minutes.
How do I order CYCV06?
Request a quote with the catalog number, quantity and destination. Single-unit orders are accepted; availability, lead time and shipping conditions are confirmed on the quotation, payment is by USD bank transfer, and items can be combined into one shipment.
Browse related products: Ptopo cloning kits.
Product manual
CYCV06 instructions for use (English, PDF).