CYCV04 is a T-vector TA cloning kit for PCR products with a 3′ A overhang, as made by Taq and Tth polymerases. The vector derives from pBlueScript II SK(+) and is cut with XcmI to leave a single unpaired 3′ T on each end, giving higher recombination efficiency. Blue background is under 10% and over 90% of white colonies carry an insert. Two EcoRI sites flanking the insert site allow a cheap EcoRI single digest to check inserts, and the vector has no NdeI or NcoI site.
Catalog no. CYCV04 · For research use only
Request a quote · Download manual (PDF)
Key features
- Vector from pBlueScript II SK(+) with unpaired 3′ T overhangs created by XcmI cutting
- Blue colonies under 10%; over 90% of white colonies carry an insert
- Fast ligation
- Two EcoRI sites flank the insert site for cheap single-enzyme insert checking; no NdeI or NcoI site, so genes with those sites can be cloned
- Vector stable to at least 10 freeze-thaw cycles at -20 °C; for research use only
Catalog numbers and pack sizes
| Catalog # | Product | Pack size |
|---|---|---|
| CYCV0401 | pBLUE-TA Cloning Kit | 20 reactions (without T4 ligase) |
| CYCV0402 | pBLUE-TA Cloning Kit | 60 reactions (without T4 ligase) |
| CYCV0403 | pBLUE-TA Cloning Kit | 20 reactions (with T4 ligase) |
| CYCV0404 | pBLUE-TA Cloning Kit | 60 reactions (with T4 ligase) |
Specifications
| Vector | pBLUE-T (from pBlueScript II SK(+)) |
|---|---|
| Insert | PCR products with 3′ A overhang (Taq, Tth) |
| Blue background | <10% |
| White colonies with insert | >90% |
| Storage | -20 °C |
| Configurations | With or without T4 DNA ligase |
| Use | Research use only |
Applications
- TA cloning of PCR products
- Blue-white screening of clones
- Quick EcoRI digest checks of inserts
Ordering
International ordering — CYCV04
- Single-unit orders are accepted, subject to confirmation — availability and lead time are confirmed on the quotation.
- Different Changyu Bio and branded items can be combined into one shipment.
- International delivery, including Europe, the United States and Canada, Brazil and Latin America, the Middle East, Africa and Asia; destination and import requirements are confirmed before shipment, and air freight typically takes 7–14 days after dispatch.
- Storage: -20 °C. Shipping temperature is confirmed per item.
- Payment by bank transfer (T/T) in US dollars after you confirm the quotation.
- Request a quotation for CYCV04 — tell us the quantity and destination city.
pBLUE-TA Cloning Kit FAQ
What PCR products can I clone?
Products with a 3′ A overhang, such as those made by Taq or Tth DNA polymerase. Blunt products from proofreading enzymes need A-tailing first (see CYCV06).
Is T4 ligase included?
It depends on the catalog number: the kit is offered both with and without T4 ligase.
How is it stored?
At -20 °C. Repeated freeze-thaw of the vector at least 10 times does not affect quality.
How do I order CYCV04?
Request a quote with the catalog number, quantity and destination. Single-unit orders are accepted; availability, lead time and shipping conditions are confirmed on the quotation, payment is by USD bank transfer, and items can be combined into one shipment.
Browse related products: Ptopo cloning kits.