CYCV13 is a zero-background blunt-end cloning kit that uses a suicide-gene positive selection system to clone blunt PCR products and other blunt DNA, phosphorylated or not. Ligation takes 5 minutes and gives over 90% positive recombinant clones. The Simple vector removes multiple cloning sites near the insert site, so restriction sites must be added on the PCR primers.
Catalog no. CYCV13 · For research use only
Request a quote · Download manual (PDF)
Key features
- Suicide-gene positive selection; no blue-white screening needed
- 5 minute ligation with over 90% positive recombinants
- Works with phosphorylated or unphosphorylated blunt DNA, including products from proofreading polymerases such as Pfu
- No multiple cloning sites near the insert site; added primer restriction sites cut without interference, improving subcloning success
- Ligation product transforms common E. coli strains directly; research use only
Catalog numbers and pack sizes
| Catalog # | Product | Pack size |
|---|---|---|
| CYCV1301 | pZERO-Blunt Simple Cloning Kit | 20 reactions |
| CYCV1302 | pZERO-Blunt Simple Cloning Kit | 40 reactions |
Specifications
| Vector | pZERO-Blunt Simple (suicide gene, no MCS) |
|---|---|
| Insert | Blunt-end DNA, phosphorylated or not |
| Ligation time | 5 minutes |
| Positive rate | >90% |
| Restriction sites | Add on PCR primers |
| Storage | -20 °C; stable for 6 months |
| Use | Research use only |
Applications
- Cloning blunt-end PCR products amplified by proofreading polymerase
- Subcloning with restriction sites added on PCR primers
- Cloning any blunt DNA fragment
Ordering
International ordering — CYCV13
- Single-unit orders are accepted, subject to confirmation — availability and lead time are confirmed on the quotation.
- Different Changyu Bio and branded items can be combined into one shipment.
- International delivery, including Europe, the United States and Canada, Brazil and Latin America, the Middle East, Africa and Asia; destination and import requirements are confirmed before shipment, and air freight typically takes 7–14 days after dispatch.
- Shipping conditions are confirmed per item on the quotation.
- Payment by bank transfer (T/T) in US dollars after you confirm the quotation.
- Request a quotation for CYCV13 — tell us the quantity and destination city.
pZERO-Blunt Simple Cloning Kit FAQ
How does the zero-background selection work?
The vector contains a lethal suicide gene. A successful insert disrupts it so recombinant cells grow, while self-ligated empty vector expresses the gene and the cells die.
Why add restriction sites on the primers?
The Simple vector removes nearby multiple cloning sites, so digestion with the primer-introduced sites is not affected by other vector sites.
Which inserts work?
Blunt-end PCR products from proofreading polymerases such as Pfu, and any blunt DNA fragment, phosphorylated or not.
How do I order CYCV13?
Request a quote with the catalog number, quantity and destination. Single-unit orders are accepted; availability, lead time and shipping conditions are confirmed on the quotation, payment is by USD bank transfer, and items can be combined into one shipment.
Browse related products: Ptopo cloning kits.