CYCV19 is a seamless cloning kit that does not rely on T4 ligase or on restriction sites. A special enzyme mix recombines a linearized vector with a PCR fragment carrying 16-25 bp of overlap at each end. In 30 minutes a 50 bp to 15 kb blunt-end PCR fragment can be inserted at any site of any vector.
Catalog no. CYCV19 · For research use only
Request a quote · Download manual (PDF)
Key features
- Recombination through 16-25 bp overlaps; no T4 ligase or phosphatase needed
- Inserts a 50 bp to 15 kb PCR fragment (blunt ends) into any vector site in 30 minutes
- Independent of restriction site availability and of blunt versus sticky ends
- Seamless: no unwanted bases added at the insertion point
- Positive clone rate above 95%; stored at -20 °C; research use only
Catalog numbers and pack sizes
| Catalog # | Product | Pack size |
|---|---|---|
| CYCV1901 | One Step Seamless Cloning Kit (Single Fragment) | 20 reactions |
Specifications
| Method | Overlap recombination, single fragment |
|---|---|
| Overlap | 16-25 bp each end |
| Insert | 50 bp – 15 kb, blunt-end PCR product |
| Time | 30 minutes |
| Positive rate | >95% |
| Storage | -20 °C, avoid repeated freeze-thaw |
| Use | Research use only |
Applications
- Inserting a PCR fragment at any vector site
- Cloning without regard to restriction sites
- Seamless construct building
Ordering
International ordering — CYCV19
- Single-unit orders are accepted, subject to confirmation — availability and lead time are confirmed on the quotation.
- Different Changyu Bio and branded items can be combined into one shipment.
- International delivery, including Europe, the United States and Canada, Brazil and Latin America, the Middle East, Africa and Asia; destination and import requirements are confirmed before shipment, and air freight typically takes 7–14 days after dispatch.
- Storage: -20 °C, avoid repeated freeze-thaw. Shipping temperature is confirmed per item.
- Payment by bank transfer (T/T) in US dollars after you confirm the quotation.
- Request a quotation for CYCV19 — tell us the quantity and destination city.
One Step Seamless Cloning Kit (Single Fragment) FAQ
Do I need restriction sites?
No. Cloning is not limited by restriction site availability or by blunt/sticky ends.
How much overlap do I design?
16-25 bp of overlap between the PCR fragment ends and the ends of the linearized vector.
How fast is it?
30 minutes, with a positive clone rate above 95%.
How do I order CYCV19?
Request a quote with the catalog number, quantity and destination. Single-unit orders are accepted; availability, lead time and shipping conditions are confirmed on the quotation, payment is by USD bank transfer, and items can be combined into one shipment.
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