CYCV21 is a TOPO cloning kit for any PCR product without 5′ phosphates, compatible with A-ends and blunt ends. It uses topoisomerase rather than T4 ligase to join DNA in seconds to minutes with close to 100% efficiency, and a suicide-gene system gives zero background, so blue-white and colony PCR screening are not needed. Vector is under 2 kb and inserts of up to 10 kb can be ligated.
Catalog no. CYCV21 · For research use only
Request a quote · Download manual (PDF)
Key features
- Topoisomerase ligation in seconds to minutes with close to 100% efficiency
- Vector smaller than 2 kb, giving more transformants and better ligation of large inserts; fragments up to 10 kb can be ligated
- Ampicillin resistance needs only 0-10 minutes recovery after transformation; recovery can be skipped for routine inserts, so ligation to plating takes about 15 minutes; for long inserts or low-efficiency competent cells, recover 30–60 minutes as the manual describes
- Suicide-gene zero-background selection; no blue-white or colony PCR screening, and a randomly picked colony nearly always has an insert
- Accepts any PCR product without 5′ phosphates, compatible with A-ends and blunt ends
- For research use only
Catalog numbers and pack sizes
| Catalog # | Product | Pack size |
|---|---|---|
| CYCV2101 | Zero Background pTOPO-TA/Blunt Cloning Kit | 40 reactions (5 μL) |
| CYCV2102 | Zero Background pTOPO-TA/Blunt Cloning Kit | 160 reactions (5 μL) |
Specifications
| Format | TOPO vector cloning kit |
|---|---|
| Insert | any PCR product without 5′ phosphates, compatible with A-ends and blunt ends |
| Ligation | Seconds to minutes |
| Max insert | 10 kb |
| Selection | Ampicillin; suicide-gene zero background |
| Sequencing primers | M13F/M13R universal primers (not M13(-47)/M13(-48)) |
| Storage | -20 °C; stable for at least 12 months |
| Shipping | Ice packs |
| Use | Research use only |
Applications
- Fast cloning of PCR products without screening
- Cloning long fragments up to 10 kb
- Routine TA or blunt-end PCR product cloning
Ordering
International ordering — CYCV21
- Single-unit orders are accepted, subject to confirmation — availability and lead time are confirmed on the quotation.
- Different Changyu Bio and branded items can be combined into one shipment.
- International delivery, including Europe, the United States and Canada, Brazil and Latin America, the Middle East, Africa and Asia; destination and import requirements are confirmed before shipment, and air freight typically takes 7–14 days after dispatch.
- Shipping conditions are confirmed per item on the quotation.
- Payment by bank transfer (T/T) in US dollars after you confirm the quotation.
- Request a quotation for CYCV21 — tell us the quantity and destination city.
Zero Background pTOPO-TA/Blunt Cloning Kit FAQ
What is the reaction format?
Each unit is a 5 μL reaction; 40 or 160 reactions per kit. At 10 μL per ligation the kit covers half as many ligations.
How do I sequence the insert?
Use M13F/M13R universal primers, not M13(-47)/M13(-48). Colony PCR can use the same primers.
Is blue-white screening needed?
No. The suicide-gene system gives zero background, so a randomly picked colony nearly always carries an insert.
How do I order CYCV21?
Request a quote with the catalog number, quantity and destination. Single-unit orders are accepted; availability, lead time and shipping conditions are confirmed on the quotation, payment is by USD bank transfer, and items can be combined into one shipment.
Browse related products: Ptopo cloning kits.