CYPC69 is a random mutagenesis kit based on error-prone PCR, introducing random mutations into a target gene fragment during amplification. Its RandomMut DNA polymerase, used with the supplied buffer and the recommended amount of Mutation enhancer, introduces about 7.8 random mutations per kb. The two directions of base change are balanced and insertions and deletions are also introduced.
Catalog no. CYPC69 · For research use only
Key features
- RandomMut DNA polymerase made for error-prone PCR
- About 7.8 random mutations per kb with the recommended Mutation enhancer
- AT to GC and GC to AT mutations balanced (ratio 1.06), with moderate insertions and deletions
- Amplifies target fragments of up to 6 kb
- Products have sticky ends and can go directly into routine T-vector cloning; stored at -20 °C for 12 months; research use only
Catalog numbers and pack sizes
| Catalog # | Product | Pack size |
|---|---|---|
| CYPC6901 | RandomMutation Random Mutagenesis Kit (Error-Prone PCR) | 50 reactions |
| CYPC6902 | RandomMutation Random Mutagenesis Kit (Error-Prone PCR) | 200 reactions |
Specifications
| Method | Error-prone PCR |
|---|---|
| Mutation rate | ~7.8 per kb (with Mutation enhancer) |
| Mutation bias | AT-to-GC / GC-to-AT = 1.06 |
| Fragment size | Up to 6 kb |
| Product ends | Sticky (A-overhang) ends for T-vector cloning |
| Storage | -20 °C, 12 months |
| Use | Research use only |
Applications
- Studying protein structure and function relationships
- Directed evolution and enzyme activity improvement
- Building mutant libraries for screening
Ordering
Single-unit orders are accepted. Quotes and invoices are in USD with payment by company bank transfer, and different items can be combined into one shipment. Send the catalog number, quantity and destination via the quote form.
RandomMutation Random Mutagenesis Kit (Error-Prone PCR) FAQ
How many mutations does it introduce?
About 7.8 random mutations per kb with the recommended Mutation enhancer. The ratio of AT-to-GC to GC-to-AT changes is 1.06, and insertions and deletions are also introduced.
How long a fragment can it amplify?
Target fragments up to 6 kb.
Can I clone the products directly?
Yes. The products have sticky ends and can be used directly in routine T-vector cloning.
How do I order CYPC69?
Request a quote with the catalog number; single-unit orders accepted, payment in USD by company transfer, and items can be combined into one shipment.
Browse related products: Ptopo cloning kits.