CYPP13 is a 100x phosphatase inhibitor cocktail, an improved version of a mainstream formula containing five separate inhibitors: sodium orthovanadate, sodium fluoride, sodium molybdate, sodium tartrate and imidazole. It strongly inhibits serine/threonine and tyrosine phosphatases, alkaline and acid phosphatases and ATPases, and is added straight to lysate to keep proteins phosphorylated.
Catalog no. CYPP13 · For research use only
Key features
- Five independent phosphatase inhibitors: sodium orthovanadate, sodium fluoride, sodium molybdate, sodium tartrate, imidazole
- Inhibits serine/threonine phosphatases, tyrosine phosphatases, alkaline and acid phosphatases and ATPases
- 100x concentrate added directly to lysate from any tissue or cell type
- Maintains protein phosphorylation for Western blot, co-IP and kinase activity assays
- For research use only
Catalog numbers and pack sizes
| Catalog # | Product | Pack size |
|---|---|---|
| CYPP1301 | Phosphatase Inhibitor Cocktail I (100x) | 1 mL |
| CYPP1302 | Phosphatase Inhibitor Cocktail I (100x) | 5 x 1 mL |
Specifications
| Format | 100x concentrated solution |
|---|---|
| Components | Sodium orthovanadate, sodium fluoride, sodium molybdate, sodium tartrate, imidazole |
| Targets | Ser/Thr and Tyr phosphatases, alkaline and acid phosphatases, ATPases |
| Storage | -20 °C, one year |
| Use | Research use only |
Applications
- Western blot detection of phosphorylated proteins
- Co-immunoprecipitation of phosphoproteins
- Protein kinase activity assays
Ordering
Single-unit orders are accepted. Quotes and invoices are in USD with payment by company bank transfer, and different items can be combined into one shipment. Send the catalog number, quantity and destination via the quote form.
Phosphatase Inhibitor Cocktail I (100x) FAQ
Why add phosphatase inhibitors?
Lysis releases endogenous phosphatases that remove phosphate groups from proteins; an external inhibitor cocktail keeps the phosphorylation state.
How is it used?
It is a 100x concentrate that can be added directly to lysate from any tissue or cell type.
How is it stored?
At -20 °C, valid for one year.
How do I order CYPP13?
Request a quote with the catalog number; single-unit orders accepted, payment in USD by company transfer, and items can be combined into one shipment.
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