CYPP12 is a RIPA (radio-immunoprecipitation assay) lysis buffer for fast lysis of cells and tissue. Its main components are 50 mM Tris (pH 7.4), 150 mM NaCl, 1% Triton X-100, 1% sodium deoxycholate and 0.1% SDS, plus phosphatase inhibitors including sodium orthovanadate, sodium fluoride and EDTA. Lysates are used for routine Western blot and IP.
Catalog no. CYPP12 · For research use only
Key features
- Traditional fast lysis buffer for cells and tissue
- 50 mM Tris pH 7.4, 150 mM NaCl, 1% Triton X-100, 1% sodium deoxycholate, 0.1% SDS
- Contains phosphatase inhibitors: sodium orthovanadate, sodium fluoride, EDTA
- Add a protease inhibitor such as PMSF before use
- For research use only
Catalog numbers and pack sizes
| Catalog # | Product | Pack size |
|---|---|---|
| CYPP1201 | RIPA Lysis Buffer | 50 mL |
| CYPP1202 | RIPA Lysis Buffer | 100 mL |
Specifications
| Format | Lysis buffer |
|---|---|
| Composition | 50 mM Tris pH 7.4, 150 mM NaCl, 1% Triton X-100, 1% DOC, 0.1% SDS |
| Inhibitors | Phosphatase inhibitors (orthovanadate, NaF, EDTA) |
| Add before use | PMSF; optionally leupeptin, aprotinin |
| Quantification | BCA assay (CYPP01); not suitable for Bradford |
| Working temperature | On ice or 4 °C |
| Use | Research use only |
Applications
- Western blotting
- Immunoprecipitation
- Total protein extraction from cells and tissue
Ordering
Single-unit orders are accepted. Quotes and invoices are in USD with payment by company bank transfer, and different items can be combined into one shipment. Send the catalog number, quantity and destination via the quote form.
RIPA Lysis Buffer FAQ
What should I add before use?
A protease inhibitor such as PMSF, and optionally leupeptin, aprotinin or others. Work on ice or at 4 °C.
Why is SDS precipitating?
SDS can precipitate at 4 °C. Redissolve completely in a 37-60 °C water bath, then return to room temperature before use.
How do I measure protein concentration?
The lysate contains a high detergent level, so the Bradford method is not suitable; use the BCA kit (CYPP01) instead.
How do I order CYPP12?
Request a quote with the catalog number; single-unit orders accepted, payment in USD by company transfer, and items can be combined into one shipment.
Browse related products: Research reagents.