CYPP07 is a Western blot stripping buffer that removes primary and secondary antibodies from a transferred membrane so it can be reprobed, for example with an Actin or Tubulin reference. It comes in two formulas for different antigen-antibody affinities, Strong buffer and Mild buffer. Reprobing the same membrane saves time and removes loading differences from running a new gel.
Catalog no. CYPP07 · For research use only
Key features
- Two formulas, Strong and Mild, for antigen-antibody complexes of different affinity
- Strong action on antibodies but gentle on bound protein; membranes can generally be reused 5 times or more
- No beta-mercaptoethanol, so no odor
- Performed at room temperature in as little as 15-20 minutes
- For research use only
Catalog numbers and pack sizes
| Catalog # | Product | Pack size |
|---|---|---|
| CYPP0701 | Stripping Buffer (Western Blot Membrane Regeneration) | 500 mL |
| CYPP07 (quote by size) | Stripping Buffer (Western Blot Membrane Regeneration) | 1 L |
Specifications
| Format | Stripping buffer, Strong and Mild formulas |
|---|---|
| Temperature | Room temperature |
| Time | As little as 15-20 minutes |
| Membrane reuse | Generally 5 times or more |
| Contains | No beta-mercaptoethanol |
| Use | Research use only |
Applications
- Reprobing a Western blot membrane with a loading control such as Actin or Tubulin
- Detecting additional proteins on the same blot
- Reducing loading variability between probes
Ordering
Single-unit orders are accepted. Quotes and invoices are in USD with payment by company bank transfer, and different items can be combined into one shipment. Send the catalog number, quantity and destination via the quote form.
Stripping Buffer (Western Blot Membrane Regeneration) FAQ
Why use two formulas?
No stripping buffer fits every antigen-antibody pair: a buffer that is too strong also removes antigen, while one that is too weak leaves antibody behind. The Strong and Mild buffers let you pick by binding strength.
How many times can a membrane be reused?
Usually 5 times or more, across several antibody detections.
Does it contain beta-mercaptoethanol?
No, so it has no odor.
How do I order CYPP07?
Request a quote with the catalog number; single-unit orders accepted, payment in USD by company transfer, and items can be combined into one shipment.
Browse related products: Research reagents.