CYPC40 is a 20x concentrate of SYBR Green I dye for real-time PCR. Its fluorescence rises 800-1000 fold on binding double-stranded DNA, so signal tracks PCR product accumulation, measured at annealing or extension. Add it to the PCR to a final concentration of 0.5x, adjustable between 0.2x and 1x.
Catalog no. CYPC40 · For research use only
Key features
- 20x concentrate; supplied as 100 μL or 1 mL
- Fluorescence increases 800-1000 fold on dsDNA binding
- Final concentration 0.2x to 1x; typical 0.5x
- Raise Mg2+ by 0.5-3 mM over a normal PCR when using SYBR Green I
- Shipped at room temperature; for research use only
Catalog numbers and pack sizes
| Catalog # | Product | Pack size |
|---|---|---|
| CYPC4001 | 20x SYBR Green I (For Real-Time PCR) | 100 μL (100 reactions of 20 μL) |
| CYPC4002 | 20x SYBR Green I (For Real-Time PCR) | 1 mL (1000 reactions of 20 μL) |
Specifications
| Format | 20x concentrate |
|---|---|
| Final concentration | 0.5x (0.2x-1x) |
| Mg2+ adjustment | 0.5-3 mM above normal PCR |
| Storage | -20 °C long term; 4 °C short term |
| Shipping | Room temperature |
| Use | Research use only |
Applications
- Preparing custom SYBR Green I real-time PCR mixes
- Dye-based quantification of PCR products
- Real-time PCR with dye optimization
Ordering
Single-unit orders are accepted. Quotes and invoices are in USD with payment by company bank transfer, and different items can be combined into one shipment. Send the catalog number, quantity and destination via the quote form.
20x SYBR Green I (For Real-Time PCR) FAQ
What final concentration should I use?
Typically 0.5x, adjustable between 0.2x and 1x. Too little dye weakens the signal change and may miss low-copy samples; too much inhibits PCR.
Do I need extra magnesium?
Raising Mg2+ lowers the inhibitory effect of SYBR Green I; use 0.5-3 mM more than in a normal PCR.
How is it stored?
At -20 °C for long-term storage, avoiding repeated freeze-thaw. Short-term use can be at 4 °C.
How do I order CYPC40?
Request a quote with the catalog number; single-unit orders accepted, payment in USD by company transfer, and items can be combined into one shipment.
Browse related products: Pcr & qpcr master mixes.