CYPC13 is a 2x ready-to-use hot-start PCR master mix containing HotMaster Taq DNA polymerase, dNTPs, MgCl2 and reaction buffer. A reversible, temperature-dependent ligand block holds the enzyme inactive below 40 °C to limit nonspecific amplification. Versions with and without dye are offered.
Catalog no. CYPC13 · For research use only
Key features
- HotMaster Taq DNA polymerase held inactive below 40 °C by a temperature-dependent inhibitory ligand
- Enzyme control continues through annealing, to limit nonspecific products throughout the run
- Contains dNTPs, MgCl2 and reaction buffer at 2x
- Only template, primers and water need to be added
- Available with or without dye; for research use only
Catalog numbers and pack sizes
| Catalog # | Product | Pack size |
|---|---|---|
| CYPC1301 | 2x HotMaster Taq PCR MasterMix | 0.5 mL (with dye) |
| CYPC1311 | 2x HotMaster Taq PCR MasterMix | 0.5 mL (without dye) |
| CYPC1302 | 2x HotMaster Taq PCR MasterMix | 5 mL (with dye) |
| CYPC1312 | 2x HotMaster Taq PCR MasterMix | 5 mL (without dye) |
Specifications
| Format | 2x master mix |
|---|---|
| Enzyme | HotMaster Taq DNA polymerase (ligand-based hot start) |
| Enzyme concentration | 0.1 U/μL |
| Components | dNTPs, MgCl2, reaction buffer |
| Storage | -20 °C, long term |
| Use | Research use only |
Applications
- Specific PCR with reduced nonspecific products
- Routine PCR amplification
- Genotyping and screening PCR
Ordering
Single-unit orders are accepted. Quotes and invoices are in USD with payment by company bank transfer, and different items can be combined into one shipment. Send the catalog number, quantity and destination via the quote form.
2x HotMaster Taq PCR MasterMix FAQ
How does the hot start work?
HotMaster Taq DNA polymerase blocks the substrate-binding site with an inhibitory ligand in a temperature-dependent way: below 40 °C an inactive enzyme-inhibitor complex forms, and at the primer annealing temperature the equilibrium shifts toward the template-primer complex.
How do I set up a reaction?
Add template and primers to the required volume of 2x mix, then add deionized water so the mix is at 1x. Dissolve and mix well first, and work on ice.
Is a dye-free version available?
Yes. Both with-dye and without-dye versions are listed.
How do I order CYPC13?
Request a quote with the catalog number; single-unit orders accepted, payment in USD by company transfer, and items can be combined into one shipment.
Browse related products: Pcr & qpcr master mixes.