CYPC86 is a 2x ready-to-use master mix for rapid PCR, containing an ultra-fast polymerase, extension enhancer, dNTPs and optimized buffer. Extension speed reaches 10 sec/kb, and about 1 sec/kb for amplicons under 1 kb. It includes electrophoresis buffer and red dye for direct gel loading.
Catalog no. CYPC86 · For research use only
Key features
- Extension speed of 5-10 sec/kb; a PCR can finish in as little as half an hour
- Fidelity stated as 3-6 times or more that of Taq
- Amplifies up to 8 kb from genomic templates and up to 12 kb from plasmid and lambda DNA; works on complex and high-GC templates
- Protectant keeps the mix stable after repeated freeze-thaw cycles
- Products carry a 3′ A overhang for T-vector cloning; contains red dye for direct loading; research use only
Catalog numbers and pack sizes
| Catalog # | Product | Pack size |
|---|---|---|
| CYPC8601 | 2x Rapid PCR MasterMix | 5 x 1 mL (with dye) |
| CYPC8602 | 2x Rapid PCR MasterMix | 15 x 1 mL (with dye) |
| CYPC8603 | 2x Rapid PCR MasterMix | 50 x 1 mL (with dye) |
Specifications
| Format | 2x master mix with electrophoresis buffer and red dye |
|---|---|
| Extension speed | 5-10 sec/kb (about 1 sec/kb under 1 kb) |
| Fidelity | 3-6x Taq or more |
| Max length | 8 kb genomic; 12 kb plasmid and lambda DNA |
| Product ends | 3′ A overhang |
| Storage | -20 °C for two years; 4 °C for use within 1 month |
| Cloning | T vectors CYCV14, CYCV15, CYCV21, CYCV22; seamless kits CYCV12, CYCV19 |
| Use | Research use only |
Applications
- Fast PCR with short extension times
- High-throughput screening PCR
- Cloning amplicons into T vectors (CYCV14, CYCV15, CYCV21, CYCV22)
- Seamless cloning workflows (CYCV12, CYCV19)
Ordering
Single-unit orders are accepted. Quotes and invoices are in USD with payment by company bank transfer, and different items can be combined into one shipment. Send the catalog number, quantity and destination via the quote form.
2x Rapid PCR MasterMix FAQ
How fast is the extension?
Extension speed reaches 5-10 sec/kb, and about 1 sec/kb for amplicons under 1 kb.
Can I load the reaction on a gel directly?
Yes. The mix contains electrophoresis buffer and red dye.
Can the product be cloned into T vectors?
Yes. Products have a 3′ A overhang and can be cloned into T vectors, including CYCV14, CYCV15, CYCV21 and CYCV22.
How do I order CYPC86?
Request a quote with the catalog number; single-unit orders accepted, payment in USD by company transfer, and items can be combined into one shipment.
Browse related products: Pcr & qpcr master mixes.