CYPC57 is a 2x ready-to-use PCR master mix containing KOD DNA polymerase, dNTPs, MgCl2 and reaction buffer. KOD polymerase has strong 3′-5′ exonuclease activity and fast synthesis of 100-138 bp/s. It suits high-fidelity amplification of products up to 6 kb, with blunt-ended output.
Catalog no. CYPC57 · For research use only
Key features
- KOD DNA polymerase from Thermococcus kodakaraensis, with strong 3′-5′ exonuclease activity
- Fidelity stated as about 50 times that of Taq and higher than Pfu
- Synthesis rate of 100-138 bp/s, stated as about 5 times Pfu and 2 times Taq
- Suited to high-fidelity products up to 6 kb; for complex genomic DNA, 2 kb or less is recommended
- Blunt-end products; mix includes dNTPs, MgCl2 and buffer; research use only
Catalog numbers and pack sizes
| Catalog # | Product | Pack size |
|---|---|---|
| CYPC5701 | 2x KOD PCR MasterMix | 1 mL |
Specifications
| Format | 2x master mix |
|---|---|
| Enzyme | KOD DNA polymerase |
| Speed | 100-138 bp/s |
| Product ends | Blunt |
| Max length | 6 kb; 2 kb for complex genomic DNA |
| Storage | -20 °C |
| Setup | On ice, dilute to 1x with deionized water |
| Use | Research use only |
Applications
- High-fidelity gene amplification for cloning
- Expression construct and mutation analysis
- Fast PCR with short extension times
Ordering
Single-unit orders are accepted. Quotes and invoices are in USD with payment by company bank transfer, and different items can be combined into one shipment. Send the catalog number, quantity and destination via the quote form.
2x KOD PCR MasterMix FAQ
How long a product can it amplify?
It is suited to high-fidelity amplification of products up to 6 kb; for complex genomic DNA, 2 kb or less is recommended.
How do I set up a reaction?
Add template and primers to the required volume of 2x mix, then add deionized water so the mix is at 1x. Mix well and work on ice.
What are the ends of the product?
Blunt, suited to cloning, expression and mutation analysis.
How do I order CYPC57?
Request a quote with the catalog number; single-unit orders accepted, payment in USD by company transfer, and items can be combined into one shipment.
Browse related products: Pcr & qpcr master mixes.