CYPC89 is a direct PCR kit for identifying yeast clones. A lysis buffer rapidly lyses yeast colonies and releases plasmid DNA, which is used as PCR template without purification, with an inhibitor-tolerant 2x Yeast PCR Mix. Products can be loaded directly for electrophoresis.
Catalog no. CYPC89 · For research use only
Key features
- Lysis buffer rapidly lyses yeast colonies
- Released yeast plasmid DNA is used as template without purification
- Inhibitor-tolerant 2x Yeast PCR Mix
- Amplification products load directly onto a gel
- For research use only
Catalog numbers and pack sizes
| Catalog # | Product | Pack size |
|---|---|---|
| CYPC8901 | DirectAmp Yeast Colony PCR Kit | 100 reactions |
| CYPC8902 | DirectAmp Yeast Colony PCR Kit | 500 reactions |
Specifications
| Format | Direct colony PCR kit |
|---|---|
| Sample | Yeast colonies |
| Template | Yeast plasmid DNA, no purification |
| Storage | 2x Yeast PCR Mix at -20 °C; others at room temperature or 4 °C |
| Short-term | 2x mix at 4 °C within 3 weeks |
| Shelf life | 12 months |
| Use | Research use only |
Applications
- Identification of yeast transformants by colony PCR
- Screening plasmid presence in yeast clones
- Gel-based clone verification
Ordering
Single-unit orders are accepted. Quotes and invoices are in USD with payment by company bank transfer, and different items can be combined into one shipment. Send the catalog number, quantity and destination via the quote form.
DirectAmp Yeast Colony PCR Kit FAQ
Is DNA purification needed?
No. DNA released by the lysis buffer is used directly as PCR template.
What storage does the kit need?
Keep the 2x Yeast PCR Mix at -20 °C and avoid repeated freeze-thaw; the rest is stored at room temperature or 4 °C. Shelf life is 12 months.
Can I run the PCR product on a gel directly?
Yes. Amplified products can be loaded directly for electrophoresis.
How do I order CYPC89?
Request a quote with the catalog number; single-unit orders accepted, payment in USD by company transfer, and items can be combined into one shipment.
Browse related products: Pcr & qpcr master mixes.