CYCF01 is recombinant human epidermal growth factor (hEGF) expressed in E. coli and supplied as a sterile lyophilized powder. It is a non-glycosylated polypeptide of 53 amino acids with a molecular weight of 6.2 kDa. Sizes range from 10 μg to 1 mg.
Catalog no. CYCF01 · For research use only
Key features
- Non-glycosylated 53-amino-acid polypeptide, 6.2 kDa, produced in an E. coli expression system
- Purity above 95.0% by SEC-HPLC and by reducing and non-reducing SDS-PAGE with silver staining
- Bioactivity: ED50 below 2 ng/mL in a dose-dependent BALB/c 3T3 proliferation assay (specific activity 5.0 x 10^5 IU/mg)
- Endotoxin below 0.1 ng/μg (1 EU/μg) by LAL; N-terminal sequence Asn-Ser-Asp-Ser-Glu
- Sterile lyophilized powder, 0.2 μm filtered; for research use only
Catalog numbers and pack sizes
| Catalog # | Product | Pack size |
|---|---|---|
| CYCF00110 | Recombinant Human EGF (hEGF) | 10 μg |
| CYCF00150 | Recombinant Human EGF (hEGF) | 50 μg |
| CYCF001100 | Recombinant Human EGF (hEGF) | 100 μg |
| CYCF001200 | Recombinant Human EGF (hEGF) | 200 μg |
| CYCF001500 | Recombinant Human EGF (hEGF) | 500 μg |
| CYCF0011000 | Recombinant Human EGF (hEGF) | 1 mg |
Specifications
| Source | E. coli, recombinant human EGF |
|---|---|
| Molecular weight | 6.2 kDa (53 amino acids, non-glycosylated) |
| Purity | >95.0% (SEC-HPLC; SDS-PAGE, silver stain) |
| Bioactivity | ED50 <2 ng/mL (BALB/c 3T3), 5.0 x 10^5 IU/mg |
| Endotoxin | <0.1 ng/μg (1 EU/μg), LAL |
| Isoelectric point | 4.0-5.0 (IEF) |
| Formulation | Lyophilized from 20 mM phosphate, 50 mM NaCl, pH 7.0 |
| Storage | Below -18 °C, at least one year (lyophilized) |
| Use | Research use only |
Applications
- Cell culture studies of epithelial cell, fibroblast and endothelial cell proliferation
- Epithelial cell culture and colony formation assays
- Growth-factor signalling and differentiation research
Ordering
Single-unit orders are accepted. Quotes and invoices are in USD with payment by company bank transfer, and different items can be combined into one shipment. Send the catalog number, quantity and destination via the quote form.
Recombinant Human EGF (hEGF) FAQ
What does EGF do in cell culture?
EGF is a mitogen for fibroblasts, epithelial cells and endothelial cells in vitro and promotes colony formation in epithelial cell culture.
How do I reconstitute it?
Dissolve the lyophilized powder in sterile ultrapure water (18 MΩ-cm) at a concentration of at least 100 μg/mL, then dilute further to the working concentration.
How should CYCF01 be stored?
The lyophilized powder keeps for at least one year below -18 °C and has been tested stable for 3 weeks at room temperature. After reconstitution it is stable for 2-7 days at 4 °C, or at least three months aliquoted below -18 °C. Avoid repeated freeze-thaw cycles.
How do I order CYCF01?
Request a quote with the catalog number; single-unit orders accepted, payment in USD by company transfer, and items can be combined into one shipment.
Browse related products: Research reagents.