First-strand cDNA synthesis kit using next-generation TRUEscript H− reverse transcriptase (molecular-evolution engineered, multi-site mutated) with enhanced extension ability and stability. Pre-mixed 5× TRUE Reaction Mix plus built-in gDNA Remover — one-step genomic DNA removal without extra DNase digestion.
Key Features
- TRUEscript H− RTase with enhanced extension ability and stability
- 5× TRUE Reaction Mix pre-mixed (RTase, RNase Inhibitor, dNTPs, buffer)
- One-step gDNA Remover eliminates genomic DNA contamination
- Flexible priming: Oligo(dT), Random Primer or gene-specific primer
Specifications
| Template | Total RNA 50 ng–5 µg; mRNA 5–500 ng per 20-µl reaction |
| Components | 5× TRUE Reaction Mix, gDNA Remover, RTase components, RNase-free H2O |
| Priming | Oligo(dT) / Random Primer / GSP supported |
| Storage | -20°C; avoid repeated freeze-thaw |
Typical Applications
- qPCR gene expression analysis
- RT-PCR and RT-qPCR templates
- cDNA library construction
- Cloning from low-abundance transcripts
Cat# CYPC1802 — For research use only (RUO). Not for diagnostic or clinical use. Contact market@changyubio.com or WhatsApp +86 18665577773 for pricing and export delivery to Africa, Southeast Asia and worldwide.