Zero-background blunt-end topoisomerase cloning kit for fragments up to 10 kb generated by high-fidelity polymerases (KOD, Pfu, Phusion) or end-polished DNA. Fast, reliable cloning without ligase.
Key Features
- Blunt-end cloning for high-fidelity polymerase products
- 5-minute topoisomerase ligation at room temperature
- Zero-background vector system
- Fragments up to 10 kb
Specifications
| Pack sizes | 20 Tests (CYCV1601) / 80 Tests (CYCV1602) |
| Components | pTOPO-Blunt Vector (30 ng/µl), 1000 bp Control, 10× Enhancer |
| Storage | -20°C; stable for 12 months |
Typical Applications
- Cloning of Pfu/KOD/Phusion PCR products
- Blunt-end fragment cloning
- High-fidelity cloning workflows
Cat# CYCV16 — For research use only (RUO). Not for diagnostic or clinical use. Contact market@changyubio.com or WhatsApp +86 18665577773 for pricing and export delivery to Africa, Southeast Asia and worldwide.