Rapid spin-column kit for high-quality genomic DNA from plant tissues, including polysaccharide- and polyphenol-rich samples. Lysis Buffer PL plus Inhibitor Removal Buffer IR remove PCR inhibitors common in plant material.
Key Features
- Optimized for plant tissues including difficult samples
- Inhibitor Removal Buffer strips polysaccharides and polyphenols
- Fast protocol — from tissue to ready-to-use DNA in under an hour
- No phenol/chloroform extraction required
Specifications
| Pack sizes | 50 Preps (CYDN1401) / 100 Preps (CYDN1402) / 200 Preps (CYDN1403) |
| Components | Lysis Buffer PL, Binding Buffer PQ, Inhibitor Removal Buffer IR, Washing Buffer WB, Elution Buffer, columns |
| Storage | RT; if buffers precipitate, warm at 55°C to dissolve before use |
| Shipping | Ambient (room temperature); no ice packs |
| Note | Add indicated ethanol to Binding/Washing buffers before first use |
Typical Applications
- Plant genomic DNA from about 100 mg fresh or 30 mg dry tissue.
- Typical yield 3–25 μg DNA per 100 mg fresh tissue (manual).
- Downstream restriction, ligation, or PCR — elution buffer has no EDTA.
Input ranges at a glance
| Standard protocol | 100 mg fresh tissue or 30 mg dry tissue, ground to powder in liquid nitrogen |
| Appendix protocol (low DNA content) | 400 mg fresh or 200 mg dry tissue in 9 ml lysis buffer |
| Typical yield | 3–25 µg DNA from 100 mg fresh tissue |
| Purity / fragment length | Typical OD260/OD280 1.7–1.9; fragment length up to 30–50 kb |
| Elution | 100 µl standard, minimum 50 µl; 300–400 µl pre-warmed water for the appendix protocol |
| Time | Under 1 hour per sample |
| Storage / pack sizes | All components RT, stable 12 months; CYDN1401 (50) · CYDN1402 (100) · CYDN1403 (200 preps) |
Limits and handling
- For laboratory research use only. Not for diagnostic or therapeutic use.
- Shipped at ambient temperature (no ice packs).
- Store at room temperature (manual: 12 months). Lysis Buffer PL or IR may precipitate when cold — warm at 55°C until dissolved, then cool to room temperature.
- Do not thaw ground plant powder before adding preheated lysis buffer. Binding buffer PQ and IR are irritating — wear gloves.
- Add the indicated ethanol to PQ and WB before first use. Eluted DNA: 2–8°C short term, −20°C long term.
Who sets these specifications
Changyu Bio manufactures Cat# CYDN14 and is responsible for the specifications on this page. Confirm pack sizes, storage, and protocol against the PDF manual before use. Quotes: market@changyubio.com or WhatsApp +86 18665577773.
Plant DNA Kit FAQ
Does the standard protocol use phenol or chloroform?
The standard protocol uses chloroform/isoamyl alcohol (24:1) extraction after lysis. An optional Tris-saturated phenol (pH 8.0)/chloroform (1:1) extraction is described for plants rich in polysaccharides and polyphenols — see the manual before applying it.
What purity and fragment length can I expect?
The manual states a typical OD260/OD280 of 1.7–1.9 and fragment lengths up to 30–50 kb, with 3–25 µg DNA from 100 mg fresh tissue. DNA works directly in PCR, Southern blot and restriction digestion.
My lysis buffer has precipitate at low temperature — is it damaged?
No. Lysis Buffer PL and Inhibitor Removal Buffer IR may precipitate in the cold; warm to 55°C until dissolved and cool to room temperature before use. Precipitate in Binding Buffer PQ after adding ethanol does not affect performance — use the supernatant.
Cat# CYDN14 — For research use only (RUO). Not for diagnostic or clinical use. Contact market@changyubio.com or WhatsApp +86 18665577773 for pricing and export delivery to Africa, Southeast Asia and worldwide.