Zero-background TA topoisomerase cloning kit for fast cloning of DNA fragments up to 10 kb generated by Taq DNA polymerase (3′-A overhang). Uses Vaccinia virus topoisomerase I strand-transfer activity for 5-minute ligation.
Key Features
- 5-minute room-temperature ligation (topoisomerase I)
- Zero-background vector system
- Clones fragments up to 10 kb with 3′-A overhangs
- Includes 1000 bp control insert for verification
Specifications
| Pack sizes | 20 rxns (CYCV1401) / 80 rxns (CYCV1402) |
| Components | pTOPO-T Vector (30 ng/µl), 1000 bp Control, 10× Enhancer |
| Storage | -20°C; stable for 12 months |
Typical Applications
- TA cloning of Taq PCR products
- Library construction
- Routine subcloning
- High-throughput cloning workflows
Cat# CYCV14 — For research use only (RUO). Not for diagnostic or clinical use. Contact market@changyubio.com or WhatsApp +86 18665577773 for pricing and export delivery to Africa, Southeast Asia and worldwide.