Stored at－20°C for 2 years
The kit contains CYF8 FastLong DNA Polymerase, dNTPs and optimized reaction buffer at a concentration of 2x. Before reaction, DNA/cDNA template, specific primer, and ddH2O should be prepared to mix the final concentration of 1×.
The Mix contains red tracer dye for quicker electrophoresis. And the PCR amplification products are with polyA end, which can be directly employed to the clone reaction with our pTOPO vector. Of course, after purified, the amplification products also can be used for other follow-up experiment, such as digestion, ligation and fluorescence sequencing.
1. High yield;
2. Fast and long amplification: 5s-10s/kb, about 10kb;
3. Super Fidelity: Up to 3 to 6 times of traditional Taq DNA polymerase;
4. Powerful amplification capacity: suitable for the amplification of complicated structure or high GC content template;