Stored at－20°C for 2 years
The kit contains CYA9 LongHiFi DNA Polymerase, dNTPs and optimized reaction buffer at a concentration of 2x. Before reaction, DNA/cDNA template, specific primer, and ddH2O should be prepared to mix the final concentration of 1×.
CYA9 kits are the mixture of super-fidelity enzymes and a variety of elongation factors which enables CYA9 to greatly improve the amplification length, speed, fidelity and the yield. It is the better choice for the amplification of long fragments, high-speed and super-fidelity.
The Mix contains red tracer dye for quicker electrophoresis. And the PCR amplification products are with blunt end, which can be directly employed to the clone reaction with our pTOPO vector. Of course, after purified, the amplification products also can be used for other follow-up experiment, such as digestion, ligation and fluorescence sequencing.
1. High yield: 0.5 or one times of Pfu;
2. Fast amplification: 3-4kb/min;
3. Super Fidelity: Up to 54 times of traditional Taq DNA polymerase;
4. Powerful amplification capacity: suitable for the amplification of less than 10kb fragments (complicated genome DNA template) and less than 15kb sequences (simple gDNA, plasmid and son on);